Cloned (Comment) | Organism |
---|---|
PCR product cloned into pET-32a(+) to construct pDH32 and transformed into Escherichia coli BL21 (DE3) to give pDH32BL for protein expression. PCR product cloned into EcoRI/HindIII of pIBR25 to construct a recombinant vector, pDHC25. The vector pDHC25 subsequently transformed into the rmbB-knockout mutant of Streptomyces peucetius DHNEO by PEG-mediated protoplast transformation | Streptomyces peucetius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Streptomyces peucetius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36500 | - |
sequence analysis | Streptomyces peucetius |
54000 | - |
recombinant enzyme | Streptomyces peucetius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces peucetius | C5J046 | ATCC 27952 | - |
Purification (Comment) | Organism |
---|---|
by cobalt-immobilized affinity chromatography | Streptomyces peucetius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-glucose | enzyme consists of a C-terminal sugar-nucleotide binding domain and an N-terminal NAD+ cofactor-binding domain | Streptomyces peucetius | dTDP-4-dehydro-6-deoxy-D-glucose + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RmbB | - |
Streptomyces peucetius |
TDP-D-glucose 4,6-dehydratase | - |
Streptomyces peucetius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | enzyme consists of an N-terminal NAD+ cofactor-binding domain and a C-terminal sugar-nucleotide binding domain | Streptomyces peucetius |
General Information | Comment | Organism |
---|---|---|
malfunction | inactivation of rmbB abolishes the production of doxorubicin while complementation of the same gene in an rmbB knockout mutant restores the doxorubicin production | Streptomyces peucetius |
physiological function | rmbB provides dTDP-4-dehydro-6-deoxy-D-glucose as a nucleotide sugar precursor for the biosynthesis of doxorubicin | Streptomyces peucetius |