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Literature summary for 4.2.1.46 extracted from

  • Singh, B.; Lee, C.B.; Sohng, J.K.
    Precursor for biosynthesis of sugar moiety of doxorubicin depends on rhamnose biosynthetic pathway in Streptomyces peucetius ATCC 27952 (2010), Appl. Microbiol. Biotechnol., 85, 1565-1574.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
PCR product cloned into pET-32a(+) to construct pDH32 and transformed into Escherichia coli BL21 (DE3) to give pDH32BL for protein expression. PCR product cloned into EcoRI/HindIII of pIBR25 to construct a recombinant vector, pDHC25. The vector pDHC25 subsequently transformed into the rmbB-knockout mutant of Streptomyces peucetius DHNEO by PEG-mediated protoplast transformation Streptomyces peucetius

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Streptomyces peucetius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36500
-
sequence analysis Streptomyces peucetius
54000
-
recombinant enzyme Streptomyces peucetius

Organism

Organism UniProt Comment Textmining
Streptomyces peucetius C5J046 ATCC 27952
-

Purification (Commentary)

Purification (Comment) Organism
by cobalt-immobilized affinity chromatography Streptomyces peucetius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dTDP-glucose enzyme consists of a C-terminal sugar-nucleotide binding domain and an N-terminal NAD+ cofactor-binding domain Streptomyces peucetius dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
?

Synonyms

Synonyms Comment Organism
RmbB
-
Streptomyces peucetius
TDP-D-glucose 4,6-dehydratase
-
Streptomyces peucetius

Cofactor

Cofactor Comment Organism Structure
NAD+ enzyme consists of an N-terminal NAD+ cofactor-binding domain and a C-terminal sugar-nucleotide binding domain Streptomyces peucetius

General Information

General Information Comment Organism
malfunction inactivation of rmbB abolishes the production of doxorubicin while complementation of the same gene in an rmbB knockout mutant restores the doxorubicin production Streptomyces peucetius
physiological function rmbB provides dTDP-4-dehydro-6-deoxy-D-glucose as a nucleotide sugar precursor for the biosynthesis of doxorubicin Streptomyces peucetius