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Literature summary for 4.2.1.45 extracted from

  • He, X.; Thorson, J.S.; Liu, H.
    Probing the coenzyme and substrate binding events of CDP-D-glucose 4,6-dehydratase: mechanistic implications (1996), Biochemistry, 35, 4721-4731.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Yersinia pseudotuberculosis

Protein Variants

Protein Variants Comment Organism
H17G mutant with increased kcat and reduced Km Yersinia pseudotuberculosis
K21I mutant with reduced Km and kcat Yersinia pseudotuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00015
-
CDP-D-glucose mutant H17G Yersinia pseudotuberculosis
0.00024
-
CDP-D-glucose mutant K21I Yersinia pseudotuberculosis
0.0003
-
CDP-D-glucose wild-type Yersinia pseudotuberculosis

Organism

Organism UniProt Comment Textmining
Yersinia pseudotuberculosis
-
Pasteurella pseudotuberculosis
-

Purification (Commentary)

Purification (Comment) Organism
mutant H17G Yersinia pseudotuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.78
-
mutant H17G Yersinia pseudotuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-D-glucose
-
Yersinia pseudotuberculosis CDP-4-dehydro-6-deoxy-D-glucose + H2O
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.33
-
CDP-D-glucose mutant K21I Yersinia pseudotuberculosis
3.69
-
CDP-D-glucose wild-type enzyme Yersinia pseudotuberculosis
4.24
-
CDP-D-glucose mutant H17G Yersinia pseudotuberculosis

Cofactor

Cofactor Comment Organism Structure
NAD+ two binding sites per enzyme molecule Yersinia pseudotuberculosis