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Literature summary for 4.2.1.39 extracted from

  • Kersters, K.; De Ley, J.
    D-Gluconate dehydratase from Alcaligenes (1975), Methods Enzymol., 42, 301-304.
    View publication on PubMed
Search for more literature for 4.2.1.39

General Stability

General Stability Organism
rather labile Alcaligenes sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Alcaligenes sp.
Co2+
-
 Alcaligenes sp.
EDTA 70% inhibition at 0.1 mM Alcaligenes sp.
EDTA
-
 Clostridium pasteurianum
Ni2+
-
 Alcaligenes sp.
p-chloromercuribenzoate 30% inhibition at 0.01 mM Alcaligenes sp.
p-chloromercuribenzoate
-
 Clostridium pasteurianum
Zn2+
-
 Alcaligenes sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
20.8
-
 D-gluconate
-
 Alcaligenes sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace Clostridium pasteurianum
Mg2+ Fe2+ best activator, Mn2+, Co2+, Mg2+ can partially replace Alcaligenes sp.
Mg2+ 100% activation at 1 mM Alcaligenes sp.
Mn2+ 50% activation at 0.1 mM, 30% activation at 1 mM Alcaligenes sp.
Mn2+ Fe2+ most effective, Mn2+, Co2+, Mg2+ can partially replace Clostridium pasteurianum
Mn2+ Fe2+ most effective, Mn2+, Co2+, Mg2+ can partially replace Alcaligenes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000
-
 thin-layer chromatography Alcaligenes sp.

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Alcaligenes sp.
-
 properties are conspiciously different from those of Clostridium pasteurianum
-
Alcaligenes sp. M250
-
 properties are conspiciously different from those of Clostridium pasteurianum
-
Aspergillus niger
-
-
-
Cereibacter sphaeroides
-
 i.e. Rhodopseudomonas sphaeroides
-
Clostridium pasteurianum
-
-
-
Clostridium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alcaligenes sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.2
-
-
 Alcaligenes sp.

Storage Stability

Storage Stability Organism
-20°C, Tris-HCl buffer, 50% glycerol, 30% loss of activity within 5 months Clostridium pasteurianum
-20°C, Tris-HCl buffer, 50% glycerol, 30% loss of activity within 5 months Alcaligenes sp.
0°C, Tris-HCl buffer, pH 8.0-8.8, MgCl2, sodium-D-gluconate, 50% loss of activity after 7 days Alcaligenes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fuconate 15% of activity with D-gluconate Alcaligenes sp. ?
-
 ?
D-fuconate 15% of activity with D-gluconate Alcaligenes sp. M250 ?
-
 ?
D-gluconate
-
 Clostridium pasteurianum 2-dehydro-3-deoxy-D-gluconate
-
 ?
D-gluconate
-
 Alcaligenes sp. 2-dehydro-3-deoxy-D-gluconate
-
 ?
D-gluconate
-
 Alcaligenes sp. M250 2-dehydro-3-deoxy-D-gluconate
-
 ?
D-xylonate 89% of activity with D-gluconate Alcaligenes sp. ?
-
 ?
D-xylonate 89% of activity with D-gluconate Alcaligenes sp. M250 ?
-
 ?
additional information
-
 Clostridium pasteurianum ?
-
 ?
additional information specific for aldonic acids with 5 or 6 carbon atoms, having hydroxyl groups at C-2 and C-3 in L-threo configuration, no activity with various other compounds tested Alcaligenes sp. ?
-
 ?
additional information specific for aldonic acids with 5 or 6 carbon atoms, having hydroxyl groups at C-2 and C-3 in L-threo configuration, no activity with various other compounds tested Alcaligenes sp. M250 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 enzyme assay at Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4 8.8
-
 Alcaligenes sp.