Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thiol | storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol | Paraburkholderia xenovorans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Paraburkholderia xenovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
mesaconate | pH 8.0, 30°C | Paraburkholderia xenovorans | |
0.1 | - |
fumarate | pH 8.0, 30°C | Paraburkholderia xenovorans | |
0.28 | - |
(S)-malate | pH 8.0, 30°C | Paraburkholderia xenovorans | |
0.52 | - |
(S)-citramalate | pH 8.0, 30°C | Paraburkholderia xenovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol | Paraburkholderia xenovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
SDS-PAGE | Paraburkholderia xenovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
mesaconate + H2O | Paraburkholderia xenovorans | mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase | (S)-citramalate | - |
? | |
mesaconate + H2O | Paraburkholderia xenovorans DSMZ 17367 / LB400 | mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase | (S)-citramalate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paraburkholderia xenovorans | Q141Z6 | - |
- |
Paraburkholderia xenovorans DSMZ 17367 / LB400 | Q141Z6 | - |
- |
Oxidation Stability | Organism |
---|---|
the enzyme is oxygen sensitive, and the aerobically measured activity of the (aerobically) purified protein is relatively low. The incubation of the enzyme with Fe2+ and thiol and following measurement of the activity under strictly anaerobic conditions leads to an 4fold increase in fumarate hydratase activity | Paraburkholderia xenovorans |
Purification (Comment) | Organism |
---|---|
- |
Paraburkholderia xenovorans |
Storage Stability | Organism |
---|---|
storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol | Paraburkholderia xenovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-citramalate | - |
Paraburkholderia xenovorans | mesaconate + H2O | - |
r | |
(S)-citramalate | - |
Paraburkholderia xenovorans DSMZ 17367 / LB400 | mesaconate + H2O | - |
r | |
(S)-malate | - |
Paraburkholderia xenovorans | fumarate + H2O | - |
r | |
(S)-malate | - |
Paraburkholderia xenovorans DSMZ 17367 / LB400 | fumarate + H2O | - |
r | |
fumarate + H2O | - |
Paraburkholderia xenovorans | (S)-malate | - |
r | |
fumarate + H2O | - |
Paraburkholderia xenovorans DSMZ 17367 / LB400 | (S)-malate | - |
r | |
mesaconate + H2O | - |
Paraburkholderia xenovorans | (S)-citramalate | - |
r | |
mesaconate + H2O | mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase | Paraburkholderia xenovorans | (S)-citramalate | - |
? | |
mesaconate + H2O | - |
Paraburkholderia xenovorans DSMZ 17367 / LB400 | (S)-citramalate | - |
r | |
mesaconate + H2O | mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase | Paraburkholderia xenovorans DSMZ 17367 / LB400 | (S)-citramalate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bxe_A3136 | - |
Paraburkholderia xenovorans |
fumarase/mesaconase | - |
Paraburkholderia xenovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay | Paraburkholderia xenovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Paraburkholderia xenovorans |
General Information | Comment | Organism |
---|---|---|
metabolism | mesaconase activity of the promiscuous fumarase/mesaconase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase | Paraburkholderia xenovorans |
physiological function | the enzyme (Bxe_A3136) is in fact a promiscuous fumarase/mesaconase. It has similar efficiencies (kcat/Km) for both fumarate and mesaconate hydration. This promiscuity is physiologically relevant, as it allows the growth of this bacterium on mesaconate as a sole carbon and energy source | Paraburkholderia xenovorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
131 | - |
(S)-citramalate | pH 8.0, 30°C | Paraburkholderia xenovorans | |
398 | - |
(S)-malate | pH 8.0, 30°C | Paraburkholderia xenovorans | |
2800 | - |
fumarate | pH 8.0, 30°C | Paraburkholderia xenovorans | |
3600 | - |
mesaconate | pH 8.0, 30°C | Paraburkholderia xenovorans |