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Literature summary for 4.2.1.34 extracted from

  • Kronen, M.; Sasikaran, J.; Berg, I.A.
    Mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans (2015), Appl. Environ. Microbiol., 81, 5632-5638 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
thiol storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol Paraburkholderia xenovorans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Paraburkholderia xenovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
mesaconate pH 8.0, 30°C Paraburkholderia xenovorans
0.1
-
fumarate pH 8.0, 30°C Paraburkholderia xenovorans
0.28
-
(S)-malate pH 8.0, 30°C Paraburkholderia xenovorans
0.52
-
(S)-citramalate pH 8.0, 30°C Paraburkholderia xenovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol Paraburkholderia xenovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
SDS-PAGE Paraburkholderia xenovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
mesaconate + H2O Paraburkholderia xenovorans mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase (S)-citramalate
-
?
mesaconate + H2O Paraburkholderia xenovorans DSMZ 17367 / LB400 mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase (S)-citramalate
-
?

Organism

Organism UniProt Comment Textmining
Paraburkholderia xenovorans Q141Z6
-
-
Paraburkholderia xenovorans DSMZ 17367 / LB400 Q141Z6
-
-

Oxidation Stability

Oxidation Stability Organism
the enzyme is oxygen sensitive, and the aerobically measured activity of the (aerobically) purified protein is relatively low. The incubation of the enzyme with Fe2+ and thiol and following measurement of the activity under strictly anaerobic conditions leads to an 4fold increase in fumarate hydratase activity Paraburkholderia xenovorans

Purification (Commentary)

Purification (Comment) Organism
-
Paraburkholderia xenovorans

Storage Stability

Storage Stability Organism
storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol Paraburkholderia xenovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-citramalate
-
Paraburkholderia xenovorans mesaconate + H2O
-
r
(S)-citramalate
-
Paraburkholderia xenovorans DSMZ 17367 / LB400 mesaconate + H2O
-
r
(S)-malate
-
Paraburkholderia xenovorans fumarate + H2O
-
r
(S)-malate
-
Paraburkholderia xenovorans DSMZ 17367 / LB400 fumarate + H2O
-
r
fumarate + H2O
-
Paraburkholderia xenovorans (S)-malate
-
r
fumarate + H2O
-
Paraburkholderia xenovorans DSMZ 17367 / LB400 (S)-malate
-
r
mesaconate + H2O
-
Paraburkholderia xenovorans (S)-citramalate
-
r
mesaconate + H2O mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase Paraburkholderia xenovorans (S)-citramalate
-
?
mesaconate + H2O
-
Paraburkholderia xenovorans DSMZ 17367 / LB400 (S)-citramalate
-
r
mesaconate + H2O mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase Paraburkholderia xenovorans DSMZ 17367 / LB400 (S)-citramalate
-
?

Synonyms

Synonyms Comment Organism
Bxe_A3136
-
Paraburkholderia xenovorans
fumarase/mesaconase
-
Paraburkholderia xenovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay Paraburkholderia xenovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Paraburkholderia xenovorans

General Information

General Information Comment Organism
metabolism mesaconase activity of the promiscuous fumarase/mesaconase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase Paraburkholderia xenovorans
physiological function the enzyme (Bxe_A3136) is in fact a promiscuous fumarase/mesaconase. It has similar efficiencies (kcat/Km) for both fumarate and mesaconate hydration. This promiscuity is physiologically relevant, as it allows the growth of this bacterium on mesaconate as a sole carbon and energy source Paraburkholderia xenovorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
131
-
(S)-citramalate pH 8.0, 30°C Paraburkholderia xenovorans
398
-
(S)-malate pH 8.0, 30°C Paraburkholderia xenovorans
2800
-
fumarate pH 8.0, 30°C Paraburkholderia xenovorans
3600
-
mesaconate pH 8.0, 30°C Paraburkholderia xenovorans