BRENDA - Enzyme Database
show all sequences of 4.2.1.34

Mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans

Kronen, M.; Sasikaran, J.; Berg, I.A.; Appl. Environ. Microbiol. 81, 5632-5638 (2015) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
thiol
storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol
Paraburkholderia xenovorans
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Paraburkholderia xenovorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
mesaconate
pH 8.0, 30°C
Paraburkholderia xenovorans
0.1
-
fumarate
pH 8.0, 30°C
Paraburkholderia xenovorans
0.28
-
(S)-malate
pH 8.0, 30°C
Paraburkholderia xenovorans
0.52
-
(S)-citramalate
pH 8.0, 30°C
Paraburkholderia xenovorans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol
Paraburkholderia xenovorans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
SDS-PAGE
Paraburkholderia xenovorans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
mesaconate + H2O
Paraburkholderia xenovorans
mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase
(S)-citramalate
-
-
?
mesaconate + H2O
Paraburkholderia xenovorans DSMZ 17367 / LB400
mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase
(S)-citramalate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Paraburkholderia xenovorans
Q141Z6
-
-
Paraburkholderia xenovorans DSMZ 17367 / LB400
Q141Z6
-
-
Oxidation Stability
Oxidation Stability
Organism
the enzyme is oxygen sensitive, and the aerobically measured activity of the (aerobically) purified protein is relatively low. The incubation of the enzyme with Fe2+ and thiol and following measurement of the activity under strictly anaerobic conditions leads to an 4fold increase in fumarate hydratase activity
Paraburkholderia xenovorans
Purification (Commentary)
Purification (Commentary)
Organism
-
Paraburkholderia xenovorans
Storage Stability
Storage Stability
Organism
storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol
Paraburkholderia xenovorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(S)-citramalate
-
746828
Paraburkholderia xenovorans
mesaconate + H2O
-
-
-
r
(S)-citramalate
-
746828
Paraburkholderia xenovorans DSMZ 17367 / LB400
mesaconate + H2O
-
-
-
r
(S)-malate
-
746828
Paraburkholderia xenovorans
fumarate + H2O
-
-
-
r
(S)-malate
-
746828
Paraburkholderia xenovorans DSMZ 17367 / LB400
fumarate + H2O
-
-
-
r
fumarate + H2O
-
746828
Paraburkholderia xenovorans
(S)-malate
-
-
-
r
fumarate + H2O
-
746828
Paraburkholderia xenovorans DSMZ 17367 / LB400
(S)-malate
-
-
-
r
mesaconate + H2O
-
746828
Paraburkholderia xenovorans
(S)-citramalate
-
-
-
r
mesaconate + H2O
mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase
746828
Paraburkholderia xenovorans
(S)-citramalate
-
-
-
?
mesaconate + H2O
-
746828
Paraburkholderia xenovorans DSMZ 17367 / LB400
(S)-citramalate
-
-
-
r
mesaconate + H2O
mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase
746828
Paraburkholderia xenovorans DSMZ 17367 / LB400
(S)-citramalate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
Bxe_A3136
-
Paraburkholderia xenovorans
fumarase/mesaconase
-
Paraburkholderia xenovorans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay
Paraburkholderia xenovorans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Paraburkholderia xenovorans
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
thiol
storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol
Paraburkholderia xenovorans
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Paraburkholderia xenovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
mesaconate
pH 8.0, 30°C
Paraburkholderia xenovorans
0.1
-
fumarate
pH 8.0, 30°C
Paraburkholderia xenovorans
0.28
-
(S)-malate
pH 8.0, 30°C
Paraburkholderia xenovorans
0.52
-
(S)-citramalate
pH 8.0, 30°C
Paraburkholderia xenovorans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol
Paraburkholderia xenovorans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
SDS-PAGE
Paraburkholderia xenovorans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
mesaconate + H2O
Paraburkholderia xenovorans
mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase
(S)-citramalate
-
-
?
mesaconate + H2O
Paraburkholderia xenovorans DSMZ 17367 / LB400
mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase
(S)-citramalate
-
-
?
Oxidation Stability (protein specific)
Oxidation Stability
Organism
the enzyme is oxygen sensitive, and the aerobically measured activity of the (aerobically) purified protein is relatively low. The incubation of the enzyme with Fe2+ and thiol and following measurement of the activity under strictly anaerobic conditions leads to an 4fold increase in fumarate hydratase activity
Paraburkholderia xenovorans
Purification (Commentary) (protein specific)
Commentary
Organism
-
Paraburkholderia xenovorans
Storage Stability (protein specific)
Storage Stability
Organism
storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol
Paraburkholderia xenovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(S)-citramalate
-
746828
Paraburkholderia xenovorans
mesaconate + H2O
-
-
-
r
(S)-citramalate
-
746828
Paraburkholderia xenovorans DSMZ 17367 / LB400
mesaconate + H2O
-
-
-
r
(S)-malate
-
746828
Paraburkholderia xenovorans
fumarate + H2O
-
-
-
r
(S)-malate
-
746828
Paraburkholderia xenovorans DSMZ 17367 / LB400
fumarate + H2O
-
-
-
r
fumarate + H2O
-
746828
Paraburkholderia xenovorans
(S)-malate
-
-
-
r
fumarate + H2O
-
746828
Paraburkholderia xenovorans DSMZ 17367 / LB400
(S)-malate
-
-
-
r
mesaconate + H2O
-
746828
Paraburkholderia xenovorans
(S)-citramalate
-
-
-
r
mesaconate + H2O
mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase
746828
Paraburkholderia xenovorans
(S)-citramalate
-
-
-
?
mesaconate + H2O
-
746828
Paraburkholderia xenovorans DSMZ 17367 / LB400
(S)-citramalate
-
-
-
r
mesaconate + H2O
mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase
746828
Paraburkholderia xenovorans DSMZ 17367 / LB400
(S)-citramalate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay
Paraburkholderia xenovorans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Paraburkholderia xenovorans
General Information
General Information
Commentary
Organism
metabolism
mesaconase activity of the promiscuous fumarase/mesaconase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase
Paraburkholderia xenovorans
physiological function
the enzyme (Bxe_A3136) is in fact a promiscuous fumarase/mesaconase. It has similar efficiencies (kcat/Km) for both fumarate and mesaconate hydration. This promiscuity is physiologically relevant, as it allows the growth of this bacterium on mesaconate as a sole carbon and energy source
Paraburkholderia xenovorans
General Information (protein specific)
General Information
Commentary
Organism
metabolism
mesaconase activity of the promiscuous fumarase/mesaconase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase
Paraburkholderia xenovorans
physiological function
the enzyme (Bxe_A3136) is in fact a promiscuous fumarase/mesaconase. It has similar efficiencies (kcat/Km) for both fumarate and mesaconate hydration. This promiscuity is physiologically relevant, as it allows the growth of this bacterium on mesaconate as a sole carbon and energy source
Paraburkholderia xenovorans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
131
-
(S)-citramalate
pH 8.0, 30°C
Paraburkholderia xenovorans
398
-
(S)-malate
pH 8.0, 30°C
Paraburkholderia xenovorans
2800
-
fumarate
pH 8.0, 30°C
Paraburkholderia xenovorans
3600
-
mesaconate
pH 8.0, 30°C
Paraburkholderia xenovorans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
131
-
(S)-citramalate
pH 8.0, 30°C
Paraburkholderia xenovorans
398
-
(S)-malate
pH 8.0, 30°C
Paraburkholderia xenovorans
2800
-
fumarate
pH 8.0, 30°C
Paraburkholderia xenovorans
3600
-
mesaconate
pH 8.0, 30°C
Paraburkholderia xenovorans
Other publictions for EC 4.2.1.34
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746828
Kronen
Mesaconase activity of class ...
Paraburkholderia xenovorans, Paraburkholderia xenovorans DSMZ 17367 / LB400
Appl. Environ. Microbiol.
81
5632-5638
2015
1
-
1
-
-
-
-
4
-
1
1
2
-
13
1
-
1
-
-
-
-
1
10
-
2
1
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
4
-
1
1
2
-
1
-
1
-
-
-
1
10
-
1
-
-
-
1
-
-
-
-
2
2
-
4
4
749072
Kronen
Mesaconase/Fumarase FumD in E ...
Escherichia coli, Escherichia coli ATCC 700728 / O157:H7, Escherichia coli W3110 / K-12
PLoS ONE
10
e0145098
2015
1
-
3
-
-
-
-
15
-
1
5
2
-
13
1
-
3
-
-
-
-
-
31
-
6
3
-
-
-
3
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
15
-
1
5
2
-
1
-
3
-
-
-
-
31
-
3
-
-
-
3
-
-
-
-
2
2
-
15
15
705588
Berg
Enzymes of the citramalate cyc ...
Rhodospirillum rubrum, Rhodospirillum rubrum 1R
Mikrobiologiia
78
22-31
2009
1
-
-
-
-
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5670
Kato
3-Methylaspartate ammonia-lyas ...
Citrobacter amalonaticus, Citrobacter freundii, Clostridium sp., Raoultella planticola, Morganella morganii, Citrobacter amalonaticus YG1002, Citrobacter freundii YG-0504, Raoultella planticola IAM 1133, Morganella morganii YG-0601
Arch. Microbiol.
168
457-463
1997
-
-
-
-
-
-
-
-
-
-
-
1
-
24
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5667
Suzuki
Properties and metabolic role ...
Aerobic bacterium
J. Biochem.
81
1917-1925
1977
-
-
-
-
-
-
1
4
-
-
1
1
-
3
1
-
-
-
-
-
1
2
6
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
1
1
-
1
-
-
-
-
1
2
6
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
5666
Wang
Purification and properties of ...
Clostridium tetanomorphum
J. Biol. Chem.
244
2516-2526
1969
1
-
-
-
-
-
4
4
-
1
1
-
-
1
-
-
1
-
-
-
-
-
5
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
4
-
4
-
1
1
-
-
-
-
1
-
-
-
-
5
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
5669
Wang
Activation of L-citramalate hy ...
Clostridium tetanomorphum
J. Biol. Chem.
244
2527-2538
1969
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5665
Blair
Assay and purification of (+)- ...
Clostridium tetanomorphum
J. Biol. Chem.
241
400-408
1966
1
-
-
-
-
-
2
1
-
2
-
-
-
1
1
-
2
-
-
-
1
-
3
-
-
-
-
-
-
1
-
-
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1
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2
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1
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1
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3
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5668
Wang
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L-Citramalate hydrolyase ...
Clostridium tetanomorphum
Methods Enzymol.
13
331-344
1964
6
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6
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4
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1
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1
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2
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