Reference to 4.2.1.34; Id = 746828 - BRENDA Enzyme Database

thiol

storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol

Paraburkholderia xenovorans

expression in Escherichia coli

Paraburkholderia xenovorans

0.03

-

mesaconate

pH 8.0, 30°C

Paraburkholderia xenovorans

0.1

-

fumarate

pH 8.0, 30°C

Paraburkholderia xenovorans

0.28

-

(S)-malate

pH 8.0, 30°C

Paraburkholderia xenovorans

0.52

-

(S)-citramalate

pH 8.0, 30°C

Paraburkholderia xenovorans

Fe2+

storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol

Paraburkholderia xenovorans

60000

-

SDS-PAGE

Paraburkholderia xenovorans

mesaconate + H2O

Paraburkholderia xenovorans

mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase

(S)-citramalate

-

?

mesaconate + H2O

Paraburkholderia xenovorans DSMZ 17367 / LB400

mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase

(S)-citramalate

-

?

Paraburkholderia xenovorans

Q141Z6

-

Paraburkholderia xenovorans DSMZ 17367 / LB400

Q141Z6

-

the enzyme is oxygen sensitive, and the aerobically measured activity of the (aerobically) purified protein is relatively low. The incubation of the enzyme with Fe2+ and thiol and following measurement of the activity under strictly anaerobic conditions leads to an 4fold increase in fumarate hydratase activity

Paraburkholderia xenovorans

-

Paraburkholderia xenovorans

storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol

Paraburkholderia xenovorans

(S)-citramalate

-

746828

Paraburkholderia xenovorans

mesaconate + H2O

-

r

(S)-citramalate

-

746828

Paraburkholderia xenovorans DSMZ 17367 / LB400

mesaconate + H2O

-

r

(S)-malate

-

746828

Paraburkholderia xenovorans

fumarate + H2O

-

r

(S)-malate

-

746828

Paraburkholderia xenovorans DSMZ 17367 / LB400

fumarate + H2O

-

r

fumarate + H2O

-

746828

Paraburkholderia xenovorans

(S)-malate

-

r

fumarate + H2O

-

746828

Paraburkholderia xenovorans DSMZ 17367 / LB400

(S)-malate

-

r

mesaconate + H2O

-

746828

Paraburkholderia xenovorans

(S)-citramalate

-

r

mesaconate + H2O

mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase

746828

Paraburkholderia xenovorans

(S)-citramalate

-

?

mesaconate + H2O

-

746828

Paraburkholderia xenovorans DSMZ 17367 / LB400

(S)-citramalate

-

r

mesaconate + H2O

mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase

746828

Paraburkholderia xenovorans DSMZ 17367 / LB400

(S)-citramalate

-

?

Bxe_A3136

-

Paraburkholderia xenovorans

fumarase/mesaconase

-

Paraburkholderia xenovorans

30

-

assay

Paraburkholderia xenovorans

8

-

assay at

Paraburkholderia xenovorans

thiol

storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol

Paraburkholderia xenovorans

expression in Escherichia coli

Paraburkholderia xenovorans

0.03

-

mesaconate

pH 8.0, 30°C

Paraburkholderia xenovorans

0.1

-

fumarate

pH 8.0, 30°C

Paraburkholderia xenovorans

0.28

-

(S)-malate

pH 8.0, 30°C

Paraburkholderia xenovorans

0.52

-

(S)-citramalate

pH 8.0, 30°C

Paraburkholderia xenovorans

Fe2+

storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol

Paraburkholderia xenovorans

60000

-

SDS-PAGE

Paraburkholderia xenovorans

mesaconate + H2O

Paraburkholderia xenovorans

mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase

(S)-citramalate

-

?

mesaconate + H2O

Paraburkholderia xenovorans DSMZ 17367 / LB400

mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase

(S)-citramalate

-

?

the enzyme is oxygen sensitive, and the aerobically measured activity of the (aerobically) purified protein is relatively low. The incubation of the enzyme with Fe2+ and thiol and following measurement of the activity under strictly anaerobic conditions leads to an 4fold increase in fumarate hydratase activity

Paraburkholderia xenovorans

-

Paraburkholderia xenovorans

storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol

Paraburkholderia xenovorans

(S)-citramalate

-

746828

Paraburkholderia xenovorans

mesaconate + H2O

-

r

(S)-citramalate

-

746828

Paraburkholderia xenovorans DSMZ 17367 / LB400

mesaconate + H2O

-

r

(S)-malate

-

746828

Paraburkholderia xenovorans

fumarate + H2O

-

r

(S)-malate

-

746828

Paraburkholderia xenovorans DSMZ 17367 / LB400

fumarate + H2O

-

r

fumarate + H2O

-

746828

Paraburkholderia xenovorans

(S)-malate

-

r

fumarate + H2O

-

746828

Paraburkholderia xenovorans DSMZ 17367 / LB400

(S)-malate

-

r

mesaconate + H2O

-

746828

Paraburkholderia xenovorans

(S)-citramalate

-

r

mesaconate + H2O

mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase

746828

Paraburkholderia xenovorans

(S)-citramalate

-

?

mesaconate + H2O

-

746828

Paraburkholderia xenovorans DSMZ 17367 / LB400

(S)-citramalate

-

r

mesaconate + H2O

mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase

746828

Paraburkholderia xenovorans DSMZ 17367 / LB400

(S)-citramalate

-

?

30

-

assay

Paraburkholderia xenovorans

8

-

assay at

Paraburkholderia xenovorans

metabolism

mesaconase activity of the promiscuous fumarase/mesaconase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase

Paraburkholderia xenovorans

physiological function

the enzyme (Bxe_A3136) is in fact a promiscuous fumarase/mesaconase. It has similar efficiencies (kcat/Km) for both fumarate and mesaconate hydration. This promiscuity is physiologically relevant, as it allows the growth of this bacterium on mesaconate as a sole carbon and energy source

Paraburkholderia xenovorans

metabolism

mesaconase activity of the promiscuous fumarase/mesaconase contributes to mesaconate utilization by Burkholderia xenovorans. Mesaconate is metabolized through its hydration to (S)-citramalate. The first reaction of the pathway, the mesaconate hydratase (mesaconase) reaction, is catalyzed by a class I fumarase. The latter compound is then metabolized to acetyl-CoA and pyruvate with the participation of two enzymes of the itaconate degradation pathway, a promiscuous itaconate-CoA transferase able to activate (S)-citramalate in addition to itaconate and (S)-citramalyl-CoA lyase

Paraburkholderia xenovorans

physiological function

the enzyme (Bxe_A3136) is in fact a promiscuous fumarase/mesaconase. It has similar efficiencies (kcat/Km) for both fumarate and mesaconate hydration. This promiscuity is physiologically relevant, as it allows the growth of this bacterium on mesaconate as a sole carbon and energy source

Paraburkholderia xenovorans

131

-

(S)-citramalate

pH 8.0, 30°C

Paraburkholderia xenovorans

398

-

(S)-malate

pH 8.0, 30°C

Paraburkholderia xenovorans

2800

-

fumarate

pH 8.0, 30°C

Paraburkholderia xenovorans

3600

-

mesaconate

pH 8.0, 30°C

Paraburkholderia xenovorans

131

-

(S)-citramalate

pH 8.0, 30°C

Paraburkholderia xenovorans

398

-

(S)-malate

pH 8.0, 30°C

Paraburkholderia xenovorans

2800

-

fumarate

pH 8.0, 30°C

Paraburkholderia xenovorans

3600

-

mesaconate

pH 8.0, 30°C

Paraburkholderia xenovorans

746828

Kronen

Mesaconase activity of class ...

Paraburkholderia xenovorans, Paraburkholderia xenovorans DSMZ 17367 / LB400

Appl. Environ. Microbiol.

81

5632-5638

2015

1

-

1

-

4

-

1

2

-

13

1

-

1

-

1

10

-

2

1

-

1

-

1

-

1

-

4

-

1

2

-

1

-

1

-

1

10

-

1

-

1

-

2

-

4

749072

Kronen

Mesaconase/Fumarase FumD in E ...

Escherichia coli, Escherichia coli ATCC 700728 / O157:H7, Escherichia coli W3110 / K-12

PLoS ONE

10

e0145098

2015

1

-

3

-

15

-

1

5

2

-

13

1

-

3

-

31

-

6

3

-

3

-

1

-

3

-

15

-

1

5

2

-

1

-

3

-

31

-

3

-

3

-

2

-

15

705588

Berg

Enzymes of the citramalate cyc ...

Rhodospirillum rubrum, Rhodospirillum rubrum 1R

Mikrobiologiia

78

22-31

2009

1

-

14

-

2

-

1

-

1

-

2

-

5670

Kato

3-Methylaspartate ammonia-lyas ...

Citrobacter amalonaticus, Citrobacter freundii, Clostridium sp., Raoultella planticola, Morganella morganii, Citrobacter amalonaticus YG1002, Citrobacter freundii YG-0504, Raoultella planticola IAM 1133, Morganella morganii YG-0601

Arch. Microbiol.

168

457-463

1997

-

1

-

24

-

12

-

1

-

12

-

5667

Suzuki

Properties and metabolic role ...

Aerobic bacterium

J. Biochem.

81

1917-1925

1977

-

1

4

-

1

-

3

1

-

1

2

6

-

4

-

1

-

4

-

1

-

1

-

1

2

6

-

4

-

5666

Wang

Purification and properties of ...

Clostridium tetanomorphum

J. Biol. Chem.

244

2516-2526

1969

1

-

4

-

1

-

1

-

1

-

5

1

-

1

-

1

-

4

-

4

-

1

-

1

-

5

1

-

1

-

5669

Wang

Activation of L-citramalate hy ...

Clostridium tetanomorphum

J. Biol. Chem.

244

2527-2538

1969

-

1

-

1

-

1

-

1

-

5665

Blair

Assay and purification of (+)- ...

Clostridium tetanomorphum

J. Biol. Chem.

241

400-408

1966

1

-

2

1

-

2

-

1

-

2

-

1

-

3

-

1

-

1

-

2

-

1

-

2

-

1

-

2

-

1

-

3

-

1

-

5668

Wang

-

L-Citramalate hydrolyase ...

Clostridium tetanomorphum

Methods Enzymol.

13

331-344

1964

6

-

1

4

-

1

-

1

-

1

-

1

2

5

1

-

2

-

2

-

6

-

1

-

4

-

4

-

1

-

1

-

1

2

5

1

-

2

-

2

-

Mesaconase activity of class I fumarase contributes to mesaconate utilization by Burkholderia xenovorans

Data extracted from this reference: