Cloned (Comment) | Organism |
---|---|
variants LeuD-1-156 and LeuD-1-168, ligated into the pETM-11 expression vector, expressed in Escherichia coli BL21(DE3) RP | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
variants LeuD-1-156 and LeuD-1-168, by sitting-drop vapour-diffusion method, crystals of LeuD-1-156 belong to the hexagonal system (space group P6122 or P6522) with up to four subunits in the asymmetric unit, whereas the crystals of LeuD-1-168 belong to the monoclinic system (space group P21) with two subunits in the asymmetric unit. Both crystals diffract to beyond 2.0 A resolution | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
variants LeuD-1-156 and LeuD-1-168, by centrifugation, on Ni-NTA column and by gel filtration, more than 95% pure | Mycobacterium tuberculosis |
Subunits | Comment | Organism |
---|---|---|
monomer | variants LeuD-1-156 and LeuD-1-168, gel-filtration | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
isopropylmalate isomerase | - |
Mycobacterium tuberculosis |