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Literature summary for 4.2.1.33 extracted from

  • Karuppasamy, M.; Geerlof, A.; Schuldt, L.; Mueller-Dieckmann, C.; Weiss, M.S.
    Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the small subunit of isopropylmalate isomerase (Rv2987c) from Mycobacterium tuberculosis (2009), Acta Crystallogr. Sect. F, 65, 136-139.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
variants LeuD-1-156 and LeuD-1-168, ligated into the pETM-11 expression vector, expressed in Escherichia coli BL21(DE3) RP Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
variants LeuD-1-156 and LeuD-1-168, by sitting-drop vapour-diffusion method, crystals of LeuD-1-156 belong to the hexagonal system (space group P6122 or P6522) with up to four subunits in the asymmetric unit, whereas the crystals of LeuD-1-168 belong to the monoclinic system (space group P21) with two subunits in the asymmetric unit. Both crystals diffract to beyond 2.0 A resolution Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
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-
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Mycobacterium tuberculosis H37Rv
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-
-

Purification (Commentary)

Purification (Comment) Organism
variants LeuD-1-156 and LeuD-1-168, by centrifugation, on Ni-NTA column and by gel filtration, more than 95% pure Mycobacterium tuberculosis

Subunits

Subunits Comment Organism
monomer variants LeuD-1-156 and LeuD-1-168, gel-filtration Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
isopropylmalate isomerase
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Mycobacterium tuberculosis