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Literature summary for 4.2.1.33 extracted from

  • Bode, R.; Birnbaum, D.
    Some properties of the leucine-biosynthesizing enzymes from Candida maltosa (1991), J. Basic Microbiol., 31, 21-26.
No PubMed abstract available
Search for more literature for 4.2.1.33

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ complete inhibition at 1 mM Candida maltosa
Co2+ 3% inhibition at 1 mM Candida maltosa
Cu2+ complete inhibition at 1 mM Candida maltosa
Fe2+ 13% inhibition at 1 mM Candida maltosa
Hg2+ complete inhibition at 1 mM Candida maltosa
Mg2+ 26% inhibition at 1 mM Candida maltosa
Mn2+ 45% inhibition at 1 mM Candida maltosa
Zn2+ complete inhibition at 1 mM Candida maltosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.75
-
 beta-carboxy-beta-hydroxyisocaproate
-
 Candida maltosa
1.79
-
 citraconate crude enzyme preparation Candida maltosa
4.57
-
 alpha-hydroxy-beta-carboxyisocaproate
-
 Candida maltosa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement Candida maltosa

Organism

Organism UniProt Comment Textmining
Candida maltosa
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme activity with citraconate 4.8-fold and 7.4-fold higher than with standard substrates Candida maltosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-hydroxy-beta-carboxyisocaproate
-
 Candida maltosa ?
-
 ?
beta-carboxy-beta-hydroxyisocaproate
-
 Candida maltosa ?
-
 ?
citraconate
-
 Candida maltosa ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 enzyme assay at Candida maltosa