Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.31 extracted from

  • Van der Werf, M.J.; Van der Tweel, W.J.J.; Hartmans, S.
    Purification and characterization of maleate hydratase from Pseudomonas pseudoalcaligenes (1993), Appl. Environ. Microbiol., 59, 2823-2829.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
No requirement
-
Pseudomonas oleovorans

General Stability

General Stability Organism
DL-malate stabilizes Pseudomonas oleovorans

Inhibitors

Inhibitors Comment Organism Structure
2,2-dimethylsuccinate Ki: 0.025 mM Pseudomonas oleovorans
CuSO4 60% inhibition at 1 mM Pseudomonas oleovorans
D-citramalate Ki: 0.083 mM Pseudomonas oleovorans
D-malate Ki: 0.63 mM Pseudomonas oleovorans
Fe2(SO4)3 67% inhibition at 1 mM Pseudomonas oleovorans
HgCl2 complete inhibition at 1 mM Pseudomonas oleovorans
iodoacetamide complete inhibition at 1 mM Pseudomonas oleovorans
additional information
-
Pseudomonas oleovorans
p-chloromercuribenzoate complete inhibition at 0.1 mM Pseudomonas oleovorans
ZnSO4 complete inhibition at 1 mM Pseudomonas oleovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
citraconate
-
Pseudomonas oleovorans
0.35
-
Maleate
-
Pseudomonas oleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
alpha1 beta1, 1 * 57000 + 1 * 24000, SDS-PAGE Pseudomonas oleovorans
57000
-
alpha1 beta1, 1 * 57000 + 1 * 24000, SDS-PAGE Pseudomonas oleovorans
89000
-
gel filtration Pseudomonas oleovorans

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas oleovorans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
108
-
-
Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dimethylmaleate 0.8% of turnover of maleate, equlibrium constant: 11.2 Pseudomonas oleovorans ?
-
?
citraconate 54% of turnover of maleate, equlibrium constant: 104 Pseudomonas oleovorans D-citramalate
-
?
maleate equilibrium constant: 2050 Pseudomonas oleovorans (R)-malate
-
?

Subunits

Subunits Comment Organism
dimer alpha1 beta1, 1 * 57000 + 1 * 24000, SDS-PAGE Pseudomonas oleovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
enzyme assay at Pseudomonas oleovorans
45
-
-
Pseudomonas oleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
enzyme assay at Pseudomonas oleovorans
8
-
-
Pseudomonas oleovorans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.025
-
2,2-dimethylsuccinate
-
Pseudomonas oleovorans
0.083
-
D-citramalate
-
Pseudomonas oleovorans