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Literature summary for 4.2.1.31 extracted from

  • Dreyer, J.L.
    Isolation and biochemical characterization of maleic-acid hydratase, an iron-requiring hydro-lyase (1985), Eur. J. Biochem., 150, 145-154.
    View publication on PubMed
Search for more literature for 4.2.1.31

Activating Compound

Activating Compound Comment Organism Structure
additional information absolute requirement for Cl-, 100% activation at 100 mM, Br-, SCN-, and NO3- can partly replace, accompanying cations relatively unimportant, S2- activates at 0.01-0.1 mM Oryctolagus cuniculus

General Stability

General Stability Organism
very unstable, Na2S stabilizes, only 15% loss of activity under anaerobic conditions Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
divalent anions
-
 Oryctolagus cuniculus
EDTA 80% inhibition at 1 mM Oryctolagus cuniculus
K-acetate weak Oryctolagus cuniculus
additional information
-
 Oryctolagus cuniculus
Na-acetate weak Oryctolagus cuniculus
NH4+-acetate weak Oryctolagus cuniculus
nitrilo-triacetic acid complete inhibition at 1 mM Oryctolagus cuniculus
o-phenanthroline 65% inhibition at 1 mM Oryctolagus cuniculus
p-chloromercuribenzoate 75% inhibition at 1 mM Oryctolagus cuniculus
phosphate 92% inhibition at 100 mM Oryctolagus cuniculus
Rb-acetate weak Oryctolagus cuniculus
RuCl3 up to 100% inhibition at 0.25 mM, dependent on sequence of addition Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Oryctolagus cuniculus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can replace Fe2+ Oryctolagus cuniculus
Fe2+ activates at 0.01-0.1 mM in the presence of Na2S and thiol, dependent on sequence of addition of compounds Oryctolagus cuniculus
additional information
-
 Oryctolagus cuniculus
Ni2+ can replace Fe2+ Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68540
-
 amino acid composition Oryctolagus cuniculus
69500
-
 1 * 69500, SDS-PAGE Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
maleate Oryctolagus cuniculus product enters citric acid cycle ?
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
 rabbit
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus

Reaction

Reaction Comment Organism Reaction ID
(R)-malate = maleate + H2O Fe-S cluster involved in catalysis, trans hydration Oryctolagus cuniculus
a

Source Tissue

Source Tissue Comment Organism Textmining
renal cortex
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
317
-
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maleate
-
 Oryctolagus cuniculus (R)-malate
-
 ?
maleate product enters citric acid cycle Oryctolagus cuniculus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 69500, SDS-PAGE Oryctolagus cuniculus
More amino acid composition Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
 enzyme assay at Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
7.4 8.6 enzyme assays between Oryctolagus cuniculus