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Literature summary for 4.2.1.30 extracted from

  • Xu, X.; Zhang, G.; Lv, B.; Yuan, Y.; Li, C.
    Recombinant glycerol dehydratase from Klebsiella pneumonia XJPD-Li: induction optimization, purification and characterization (2011), Appl. Biochem. Microbiol., 47, 144-150.
No PubMed abstract available
Search for more literature for 4.2.1.30

Activating Compound

Activating Compound Comment Organism Structure
coenzyme B12 GDHt utilizes adenosylcobalamin, i.e. AdoCbl or CoB12, as a cofactor, Km is 10.03 nM Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
genes dhaBCE, recombinant expression of His6-tagged GDHt in Escherichia coli strain Bl21(DE3), optimization of IPTG inducting process for recombinant GDHt to increase specific activity and ratio of soluble form using 0.8 mM IPTG at 20°C for 3 h, method development, overview Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.48
-
 glycerol pH 8.0, 37°C Klebsiella pneumoniae
1.43
-
 1,2-propanediol pH 8.0, 37°C Klebsiella pneumoniae
3.07
-
 1,2-ethanediol pH 8.0, 37°C Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae A6NA24 large subunit, gene dhaB; gene dhaB
-
Klebsiella pneumoniae A6NA25 medium subunit, gene dhaC; gene dhaC
-
Klebsiella pneumoniae Q59475 gamma subunit, gene dhaB3 or dhaE; gene dhaB3 or dhaE
-
Klebsiella pneumoniae XJPD-Li A6NA24 large subunit, gene dhaB; gene dhaB
-
Klebsiella pneumoniae XJPD-Li A6NA25 medium subunit, gene dhaC; gene dhaC
-
Klebsiella pneumoniae XJPD-Li Q59475 gamma subunit, gene dhaB3 or dhaE; gene dhaB3 or dhaE
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged GDHt 2.11fold by nickel affinity chromatography and ultrafiltration Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-ethanediol
-
 Klebsiella pneumoniae acetaldehyde + H2O
-
 ?
1,2-ethanediol
-
 Klebsiella pneumoniae XJPD-Li acetaldehyde + H2O
-
 ?
1,2-propanediol
-
 Klebsiella pneumoniae propionaldehyde + H2O
-
 ?
1,2-propanediol
-
 Klebsiella pneumoniae XJPD-Li propionaldehyde + H2O
-
 ?
glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
 ?
glycerol
-
 Klebsiella pneumoniae XJPD-Li 3-hydroxypropanal + H2O
-
 ?

Synonyms

Synonyms Comment Organism
GDHt
-
 Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 recombinant enzyme Klebsiella pneumoniae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 45 assay range Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 recombinant enzyme Klebsiella pneumoniae

General Information

General Information Comment Organism
metabolism GDHt is the key enzyme and catalyzes the rate limiting step for biotransformation from glycerol to 1,3-propanediol Klebsiella pneumoniae