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Literature summary for 4.2.1.30 extracted from

  • Xu, X.; Zhang, G.; Wang, L.; Ma, B.; Li, C.
    Quantitative analysis on inactivation and reactivation of recombinant glycerol dehydratase from Klebsiella pneumoniae XJPD-Li (2009), J. Mol. Catal. B, 56, 108-114.
No PubMed abstract available
Search for more literature for 4.2.1.30

Activating Compound

Activating Compound Comment Organism Structure
ATP is critical to the reactivation of glycerol dehydratase Klebsiella pneumoniae
coenzyme B12 is critical to the reactivation of glycerol dehydratase Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
plasmid PKRX-T bearing the gene of the dhaBCE double digested with EcoR I and Sac I, resulting 2.7 kb fragment containing the intact dhaBCE genes inserted into pET-28a (+) vector linearized with the same enzymes. Transformation of the ligation mixture (pET-28 (+) dhaBCE) into Escherichia coli BL21 (DE3) for overexpression Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
glycerol can inactive the glycerol dehydratase expressed in Escherichia coli quickly in 10 min. Can be effectively reactivated to 98.9% by 50 mM ATP, 10 mM Mg2+ and 0.003 mM coenzyme B12, respectively, when the ratio (w/w) of glycerol dehydratase to reactivation factor is 4:1. Activity of glycerol inactivated GDHt is easier to be recovered than that of O2-inactivated GDHt Klebsiella pneumoniae
O2 can inactive the glycerol dehydratase expressed in Escherichia coli quickly in 10 min. Can be effectively reactivated to 97.3% by 50 mM ATP, 10 mM Mg2+ and 0.003 mM coenzyme B12, respectively, when the ratio (w/w) of glycerol dehydratase to reactivation factor is 4:1. Activity of glycerol inactivated GDHt is easier to be recovered than that of O2-inactivated GDHt Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16101
-
 x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis Klebsiella pneumoniae
21322
-
 x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis Klebsiella pneumoniae
60702
-
 x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae Q59475 and A6NA24 and A6NA25 Q59475: small subunit, A6NA24: large subunit, A6NA25: small subunit
-
Klebsiella pneumoniae XJPD-Li Q59475 and A6NA24 and A6NA25 Q59475: small subunit, A6NA24: large subunit, A6NA25: small subunit
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.299
-
 recombined glycerol dehydratase Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-propanediol
-
 Klebsiella pneumoniae propionaldehyde + H2O
-
 ?
1,2-propanediol
-
 Klebsiella pneumoniae XJPD-Li propionaldehyde + H2O
-
 ?
glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
 ?
glycerol
-
 Klebsiella pneumoniae XJPD-Li 3-hydroxypropanal + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 60702 + x * 21322 + x * 16101, alphabetagamma, sequence analysis Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
DhaBCE
-
 Klebsiella pneumoniae
GDHt
-
 Klebsiella pneumoniae
glycerol dehydratase
-
 Klebsiella pneumoniae

General Information

General Information Comment Organism
physiological function overexpressed glycerol dehydratase shows higher enzyme activity than that of wild-strain Klebsiella pneumoniae