Activating Compound | Comment | Organism | Structure |
---|---|---|---|
adenosylcobalamin | bound at the interface between the alpha and beta subunits | Klebsiella pneumoniae |
Crystallization (Comment) | Organism |
---|---|
complexed with cyanocobalamin an propane-1,2-diol, sandwich-drop vapor diffusion method | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
Propane-1,2-diol | 37°C, recombinant enzyme, purification method 1 and 2 | Klebsiella pneumoniae | |
0.36 | - |
Propane-1,2-diol | 37°C | Klebsiella pneumoniae | |
1.1 | - |
glycerol | 37°C, recombinant enzyme, purification method 2 | Klebsiella pneumoniae | |
1.2 | - |
glycerol | 37°C, recombinant enzyme, purification method 1 | Klebsiella pneumoniae | |
1.5 | - |
glycerol | 37°C | Klebsiella pneumoniae | |
8 | - |
adenosylcobalamin | 37°C, recombinant enzyme, purification method 1 and 2 | Klebsiella pneumoniae | |
20 | - |
adenosylcobalamin | 37°C | Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | essential, bound inside the(beta/alpha)8 barrel above the corrin ring of cobalamin | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
gamma-subunit, SDS-PAGE | Klebsiella pneumoniae |
22000 | - |
beta-subunit, SDS-PAGE | Klebsiella pneumoniae |
61000 | - |
alpha-subunit, SDS-PAGE | Klebsiella pneumoniae |
200000 | - |
native PAGE, 2 bands: 200000 Da and 220000 Da | Klebsiella pneumoniae |
220000 | - |
native PAGE, 2 bands: 200000 Da and 220000 Da | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity, recombinant enzyme, 2 different purification methods | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
65.4 | 120 | dependent on purification method | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol | - |
Klebsiella pneumoniae | 3-hydroxypropanal + H2O | - |
? | |
propane-1,2-diol | (R)-enantiomer is used 2.5 times faster than the (S)-isomer | Klebsiella pneumoniae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha2beta2gamma2, native PAGE and SDS-PAGE | Klebsiella pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
392 | - |
Propane-1,2-diol | 37°C, recombinant enzyme, purification method 2 | Klebsiella pneumoniae | |
860 | - |
glycerol | 37°C, recombinant enzyme, purification method 2 | Klebsiella pneumoniae |