Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.30 extracted from

  • Yamanishi, M.; Yunoki, M.; Tobimatsu, T.; Sato, H.; Matsui, J.; Dokiya, A.; Iuchi, Y.; Oe, K.; Suto, K.; Shibata, N.; Morimoto, Y.; Yasuoka, N.; Toraya, T.
    The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol (2002), Eur. J. Biochem., 269, 4484-4494.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
adenosylcobalamin bound at the interface between the alpha and beta subunits Klebsiella pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
complexed with cyanocobalamin an propane-1,2-diol, sandwich-drop vapor diffusion method Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
Propane-1,2-diol 37°C, recombinant enzyme, purification method 1 and 2 Klebsiella pneumoniae
0.36
-
Propane-1,2-diol 37°C Klebsiella pneumoniae
1.1
-
glycerol 37°C, recombinant enzyme, purification method 2 Klebsiella pneumoniae
1.2
-
glycerol 37°C, recombinant enzyme, purification method 1 Klebsiella pneumoniae
1.5
-
glycerol 37°C Klebsiella pneumoniae
8
-
adenosylcobalamin 37°C, recombinant enzyme, purification method 1 and 2 Klebsiella pneumoniae
20
-
adenosylcobalamin 37°C Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
K+ essential, bound inside the(beta/alpha)8 barrel above the corrin ring of cobalamin Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
gamma-subunit, SDS-PAGE Klebsiella pneumoniae
22000
-
beta-subunit, SDS-PAGE Klebsiella pneumoniae
61000
-
alpha-subunit, SDS-PAGE Klebsiella pneumoniae
200000
-
native PAGE, 2 bands: 200000 Da and 220000 Da Klebsiella pneumoniae
220000
-
native PAGE, 2 bands: 200000 Da and 220000 Da Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity, recombinant enzyme, 2 different purification methods Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
65.4 120 dependent on purification method Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol
-
Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
?
propane-1,2-diol (R)-enantiomer is used 2.5 times faster than the (S)-isomer Klebsiella pneumoniae ?
-
?

Subunits

Subunits Comment Organism
hexamer alpha2beta2gamma2, native PAGE and SDS-PAGE Klebsiella pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
392
-
Propane-1,2-diol 37°C, recombinant enzyme, purification method 2 Klebsiella pneumoniae
860
-
glycerol 37°C, recombinant enzyme, purification method 2 Klebsiella pneumoniae