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Literature summary for 4.2.1.30 extracted from

  • Macis, L.; Daniel, R.; Gottschalk, G.
    Properties and sequence of the coenzyme B12-dependent glycerol dehydratase of Clostridium pasteurianum (1998), FEMS Microbiol. Lett., 164, 21-28.
    View publication on PubMed
Search for more literature for 4.2.1.30

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Clostridium pasteurianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
190000
-
 non-denaturing PAGE Clostridium pasteurianum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol Clostridium pasteurianum
-
 ?
-
 ?
glycerol Citrobacter freundii
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Citrobacter freundii
-
 DSM 30040
-
Clostridium pasteurianum
-
 DSM 525
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Clostridium pasteurianum
additional information
-
-
 Citrobacter freundii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-ethanediol
-
 Clostridium pasteurianum acetaldehyde + H2O
-
 ?
1,2-ethanediol
-
 Citrobacter freundii acetaldehyde + H2O
-
 ?
1,2-propanediol
-
 Clostridium pasteurianum propionaldehyde + H2O
-
 ?
1,2-propanediol
-
 Citrobacter freundii propionaldehyde + H2O
-
 ?
glycerol preferred substrate Clostridium pasteurianum 3-hydroxypropanal + H2O 3-hydroxypropionaldehyde ?
glycerol preferred substrate Citrobacter freundii 3-hydroxypropanal + H2O 3-hydroxypropionaldehyde ?
glycerol
-
 Clostridium pasteurianum ?
-
 ?
glycerol
-
 Citrobacter freundii ?
-
 ?

Subunits

Subunits Comment Organism
More three structural subunits with molecular masses of 60813, 19549 and 16722 Da, calculation from sequence of amino acid Clostridium pasteurianum

Cofactor

Cofactor Comment Organism Structure
5,6-Dimethylbenzimidazolylcobamide coenzyme required Clostridium pasteurianum
5,6-Dimethylbenzimidazolylcobamide coenzyme required Citrobacter freundii