Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 0.1 mM AgNO3, apoenzyme: 97% loss of activity, inactive enzyme complex with hydroxocobalamin: 19% loss of activity | Klebsiella pneumoniae | |
EDTA | inhibition of the apoenzyme can be completely reversed by Mg2+ in adequate concentrations. Ca2+, Mn2+, Zn2+ and Co2+ also reverse the EDTA inhibitory effect. Glycerol partially reverses the inhibition. Complete inhibition of the enzyme complex with hydroxocobalamin is reversible with an excess of Mg2+ | Klebsiella pneumoniae | |
hexadecyltrimethylammonium bromide | 0.14%, complete irreversible inactivation of the apoenzyme | Klebsiella pneumoniae | |
Hg2+ | 0.1 mM HgCl2, apoenzyme: complete loss of activity, inactive enzyme complex with hydroxocobalamin: 70% loss of activity | Klebsiella pneumoniae | |
additional information | - |
Klebsiella pneumoniae | |
p-chloromercuribenzoate | 0.1 mM, apoenzyme: complete loss of activity, inactive enzyme complex with hydroxocobalamin: 70% loss of activity | Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for the reactivation of the active holoenzyme, optimal concentration: 30 mM | Klebsiella pneumoniae | |
SO32- | K2SO3, absolute requirement in the reactivation of the inactive enzyme complex with hydroxocobalamin | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
188000 | - |
sucrose density gradient centrifugation, inactive enzyme complex with hydroxocobalamin | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol | Klebsiella pneumoniae | - |
? | - |
? | |
glycerol | Klebsiella pneumoniae PZH 572 Warsaw | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae PZH 572 Warsaw | - |
- |
- |
Purification (Comment) | Organism |
---|---|
inactive enzyme complex with hydroxocobalamin | Klebsiella pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Klebsiella pneumoniae |
Storage Stability | Organism |
---|---|
stable after lyophilization | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol | - |
Klebsiella pneumoniae | 3-hydroxypropanal + H2O | beta-hydroxypropionaldehyde | ? | |
glycerol | - |
Klebsiella pneumoniae PZH 572 Warsaw | 3-hydroxypropanal + H2O | beta-hydroxypropionaldehyde | ? | |
glycerol | - |
Klebsiella pneumoniae | ? | - |
? | |
glycerol | - |
Klebsiella pneumoniae PZH 572 Warsaw | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | enzyme dissociates reversibly by action of EDTA into two different very unstable subunits, A and B | Klebsiella pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
inactive enzyme complex with hydroxocobalamin: quite stable | Klebsiella pneumoniae |
78 | - |
K+ stabilizes inactive enzyme complex with hydroxocobalamin | Klebsiella pneumoniae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | inactive enzyme complex with hydroxocobalamin, quite stable between pH 5.0 to pH 9.5, below pH 5.0: inactive enzyme complex with hydroxocobalamin dissociates irreversibly, liberating hydroxocobalamin, B12OH | Klebsiella pneumoniae |
8 | 9 | apoenzyme is unstable below pH 8.0 and above pH 9.0 | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6-Dimethylbenzimidazolylcobamide | coenzyme required | Klebsiella pneumoniae | |
5,6-dimethylbenzimidazolylcobamide 5'-phosphate | i.e. DBCC, the inactive enzyme complex with hydroxocobalamin can be converted to the active holoenzyme by replacement of hydroxocobalamin with DBCC in the presence of MG2+ and SO32- | Klebsiella pneumoniae |