Literature summary for 4.2.1.3 extracted from

Eprintsev, A.T.; Fedorin, D.N.; Nikitina, M.V.; Igamberdiev, A.U.
Expression and properties of the mitochondrial and cytosolic forms of aconitase in maize scutellum (2015), J. Plant Physiol., 181, 14-19 .

Search for more literature for 4.2.1.3

Inhibitors

Inhibitors	Comment	Organism	Structure
fumarate	-	Zea mays
glyoxylate	-	Zea mays
malate	-	Zea mays
succinate	-	Zea mays
trans-aconitate	-	Zea mays

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.49	-	isocitrate	isoform Aco1, pH 8.0, 25°C	Zea mays
1.68	-	isocitrate	isoform Aco4, pH 8.0, 25°C	Zea mays
9.5	-	citrate	isoform Aco4, pH 8.0, 25°C	Zea mays
21.1	-	citrate	isoform Aco1, pH 8.0, 25°C	Zea mays

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	isoform Aco1	Zea mays	5829	-
cytosol	isoform Aco4	Zea mays	5829	-

Organism

Organism	UniProt	Comment	Textmining
Zea mays	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
both isoforms Aco1 and Aco4	Zea mays

Source Tissue

Source Tissue	Comment	Organism	Textmining
scutellum	highest activity of both forms of aconitase on the 4th day of germination, then the activity and expression of the cytosolic form sharply decreases, while those of the mitochondrial form decrease more slowly	Zea mays	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.95	-	isoform Aco4, pH 8.0, 25°C	Zea mays
4.06	-	isoform Aco1, pH 8.0, 25°C	Zea mays

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
citrate	-	Zea mays	isocitrate	-	r
isocitrate	-	Zea mays	citrate	-	r

Synonyms

Synonyms	Comment	Organism
Aco1	mitochondrial isoform	Zea mays
ACO4	cytosolic isoform	Zea mays

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	mitchondrial isoform Aco1	Zea mays
8	-	cytosolic isoform Aco4	Zea mays

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.18	-	trans-aconitate	substrate isocitrate, isoform Aco1, pH 8.0, 25°C	Zea mays
0.25	-	trans-aconitate	substrate isocitrate, isoform Aco4, pH 8.0, 25°C	Zea mays
0.26	-	trans-aconitate	substrate citrate, isoform Aco1, pH 8.0, 25°C	Zea mays
0.39	-	trans-aconitate	substrate citrate, isoform Aco4, pH 8.0, 25°C	Zea mays
0.41	-	fumarate	isoform Aco1, pH 8.0, 25°C	Zea mays
0.58	-	succinate	isoform Aco1, pH 8.0, 25°C	Zea mays
0.58	-	fumarate	isoform Aco4, pH 8.0, 25°C	Zea mays
0.68	-	glyoxylate	isoform Aco1, pH 8.0, 25°C	Zea mays
0.68	-	succinate	isoform Aco4, pH 8.0, 25°C	Zea mays
0.71	-	malate	isoform Aco1, pH 8.0, 25°C	Zea mays
0.72	-	glyoxylate	isoform Aco4, pH 8.0, 25°C	Zea mays
0.95	-	malate	isoform Aco4, pH 8.0, 25°C	Zea mays

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Release 2023.1 (January 2023)