Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.3 extracted from

  • Gupta, P.; Ghosalkar, A.; Mishra, S.; Chaudhuri, T.K.
    Enhancement of over expression and chaperone assisted yield of folded recombinant aconitase in Escherichia coli in bioreactor cultures (2009), J. Biosci. Bioeng., 107, 102-107.
    View publication on PubMed

Application

Application Comment Organism
biotechnology molecular chaperones GroEL/GroES are co-expressed with soluble, biologically active recombinant aconitase in Escherichia coli by cultivation in a bioreactor at different temperatures under optimized conditions. The yield of functional aconitase is enhanced, either in presence of co-expressed GroEL/ES or at low temperature cultivation. The outcome from the chaperone assisted folding of aconitase is more pronounced at lower temperature Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
yeast mitochondrial aconitase is expressed in Escherichia coli by cultivation in a bioreactor at different temperatures. Chaperones GroEL and GroES are co-expressed Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate
-
Saccharomyces cerevisiae cis-aconitate + H2O
-
?

Synonyms

Synonyms Comment Organism
aconitase
-
Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Saccharomyces cerevisiae