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Literature summary for 4.2.1.3 extracted from

  • Jordan, P.A.; Tang, Y.; Bradbury, A.J.; Thomson, A.J.; Guest, J.R.
    Biochemical and spectroscopic characterization of Escherichia coli aconitases (AcnA and AcnB) (1999), Biochem. J., 344, 739-746.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information two values are obtained for KM-value with isocitrate as substrate, depending on substrate concentration Escherichia coli
0.016
-
cis-aconitate 20°C, pH 7.4, enzyme form AcnB Escherichia coli
0.058
-
cis-aconitate 20°C, pH 7.4, enzyme form AcnA Escherichia coli
1.16
-
citrate 20°C, pH 7.4, enzyme form AcnA Escherichia coli
11
-
citrate 20°C, pH 7.4, enzyme form AcnB Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Iron a significant proportion of the enzyme is in the inactive [3Fe-4S]1+ or apoenzyme forms. AcnB contains a much higher proportion of inactive enzyme than AcnA Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
and strain JRG3171, contains two major aconitases, AcnA and AcnB
-
Escherichia coli JRG2387
-
and strain JRG3171, contains two major aconitases, AcnA and AcnB
-

Purification (Commentary)

Purification (Comment) Organism
AcnA and AcnB Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-aconitate + H2O
-
Escherichia coli ?
-
?
cis-aconitate + H2O
-
Escherichia coli JRG2387 ?
-
?
citrate
-
Escherichia coli cis-aconitate + H2O
-
?
citrate
-
Escherichia coli JRG2387 cis-aconitate + H2O
-
?
isocitrate
-
Escherichia coli cis-aconitate + H2O
-
?
isocitrate
-
Escherichia coli JRG2387 cis-aconitate + H2O
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
enzyme form AcnA and AcnB, the maximal activity of AcnB is nearly 2fold the activity of AcnA Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5 10 pH 5.0: about 75% of maximal activity, pH 10.0: about 60% of maximal activity, AcnA Escherichia coli
6 9 pH 6.0: about 55% of maximal activity, pH 9.0: about 20% of maximal activity, AcnB Escherichia coli