Cloned (Comment) | Organism |
---|---|
recombinant TgPBGS is expressed in Escherichia coli as a His-tagged fusion protein | Toxoplasma gondii |
Protein Variants | Comment | Organism |
---|---|---|
DELTA646-658 | a mutant enzyme lacking the C-terminal 13 amino acids distinguishing parasite PBGS from plant and animal enzymes is purified as a dimer, suggesting that the C-terminus is required for octamer stabilisation | Toxoplasma gondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
succinylacetone | - |
Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
5-aminolevulinic acid | - |
Toxoplasma gondii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
apicoplast | - |
Toxoplasma gondii | 20011 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | enzyme responds to Mg2+ but not to Zn2+, enzyme shows two Mg2+ affinities | Toxoplasma gondii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
recombinant TgPBGS also shows low levels of dimers, 2 * 40000 Da, SDS-PAGE | Toxoplasma gondii |
40000 | - |
recombinant TgPBGS is purified as a stable octamer, 8 * 40000 Da, SDS-PAGE | Toxoplasma gondii |
320000 | - |
gel filtration, recombinant TgPBGS is purified as a stable octamer | Toxoplasma gondii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Toxoplasma gondii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.4 | - |
- |
Toxoplasma gondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aminolevulinic acid + 5-aminolevulinic acid | - |
Toxoplasma gondii | porphobilinogen + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | recombinant TgPBGS also shows low levels of dimers, 2 * 40000 Da, SDS-PAGE | Toxoplasma gondii |
octamer | recombinant TgPBGS is purified as a stable octamer, 8 * 40000 Da, SDS-PAGE | Toxoplasma gondii |
Synonyms | Comment | Organism |
---|---|---|
Porphobilinogen synthase | - |
Toxoplasma gondii |
TgPBGS | - |
Toxoplasma gondii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Toxoplasma gondii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | - |
Toxoplasma gondii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.001 | - |
- |
Toxoplasma gondii | succinylacetone |