Literature summary for 4.2.1.24 extracted from

Dhanasekaran, S.; Chandra, N.R.; Chandrasekhar Sagar, B.K.; Rangarajan, P.N.; Padmanaban, G.
delta-Aminolevulinic acid dehydratase from Plasmodium falciparum: indigenous versus imported (2004), J. Biol. Chem., 279, 6934-6942.

Search for more literature for 4.2.1.24

Cloned(Commentary)

Cloned (Comment)	Organism
very poor expression of full-length cDNA, overexpression of cDNA-2 (56 to 451 aa) as a protein with a histidine tag or as a GST fusion protein in Escherichia coli	Plasmodium falciparum

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	5 mM, 90% inhibition	Mus musculus
additional information	no inhibition by EDTA even at 25 mM	Plasmodium falciparum
succinylacetone	50% inhibition by 125 nM	Mus musculus
succinylacetone	50% inhibition by 250 nM	Plasmodium falciparum
Zn2+	pH 7.5, 50% inhibition at 0.12 mM	Mus musculus
Zn2+	at pH 8.5, 50% inhibition by 0.075 mM. At pH 7.5, 50% inhibition by less than 0.02 mM	Plasmodium falciparum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.208	-	5-aminolevulinate	pH 8.5	Plasmodium falciparum
0.294	-	5-aminolevulinate	pH 7.5	Mus musculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
apicoplast	-	Mus musculus	20011	-
apicoplast	-	Plasmodium falciparum	20011	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	stimulates, between pH 6.5 and 7.0, K+ is as stimulatory as Mg2+ and the stimulation is almost 2fold	Plasmodium falciparum
Mg2+	20-30% stimulation at pH 8.5, no requirement for a metal ion	Plasmodium falciparum
additional information	no stimulation by Mg2+	Mus musculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	8 * 38000, mature recombinant enzyme, SDS-PAGE	Mus musculus
46000	-	8 * 46000, mature recombinant enzyme, SDS-PAGE	Plasmodium falciparum
335000	-	gel filtration	Mus musculus
345000	-	gel filtration	Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5-aminolevulinate + 5-aminolevulinate	Plasmodium falciparum	the role of the enzyme may be confined to heme synthesis in the apicoplast that may not account for the total de novo heme biosynthesis in the parasite	porphobilinogen + 2 H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-
Plasmodium falciparum	-	the Plasmodium falciparum enzyme may account for about 10% of the total delta-aminolevulinate dehydratase activity in the parasite, the rest being accounted for by the host enzyme imported by the parasite	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-aminolevulinate + 5-aminolevulinate	-	Mus musculus	porphobilinogen + 2 H2O	-	?
5-aminolevulinate + 5-aminolevulinate	-	Plasmodium falciparum	porphobilinogen + 2 H2O	-	?
5-aminolevulinate + 5-aminolevulinate	the role of the enzyme may be confined to heme synthesis in the apicoplast that may not account for the total de novo heme biosynthesis in the parasite	Plasmodium falciparum	porphobilinogen + 2 H2O	-	?

Subunits

Subunits	Comment	Organism
octamer	8 * 38000, mature recombinant enzyme, SDS-PAGE	Mus musculus
octamer	8 * 46000, mature recombinant enzyme, SDS-PAGE	Plasmodium falciparum

Synonyms

Synonyms	Comment	Organism
ALAD	-	Mus musculus
ALAD	-	Plasmodium falciparum
PfALAD	-	Plasmodium falciparum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	8.5	-	Mus musculus
8	9	-	Plasmodium falciparum

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	9.5	pH 6.5: about 80% of maximal activity, pH 9.5: about 45% of maximal activity	Mus musculus
7	10	pH 7.0: about 50% of maximal activity, pH 10.0: about 55% of maximal activity	Plasmodium falciparum

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Release 2023.1 (January 2023)