Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | required at very low concentrations of substrate | Rhodobacter capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | no inhibition | Rhodobacter capsulatus | |
iodoacetamide | - |
Rhodobacter capsulatus | |
additional information | insensitive to inhibition by hemin and protoporphyrin | Rhodobacter capsulatus | |
NaCN | in presence of the substrate | Rhodobacter capsulatus | |
NEM | - |
Rhodobacter capsulatus | |
PCMB | - |
Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
5-aminolevulinate | in presence of 2-mercaptoethanol | Rhodobacter capsulatus | |
2 | - |
5-aminolevulinate | in absence of 2-mercaptoethanol | Rhodobacter capsulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | does not require metallic cations for activation | Rhodobacter capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
260000 | - |
sucrose density gradient centrifugation | Rhodobacter capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodobacter capsulatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 5-aminolevulinate = porphobilinogen + 2 H2O | substrate is covalently bound through a Schiff base | Rhodobacter capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.62 | - |
- |
Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aminolevulinate + 5-aminolevulinate | - |
Rhodobacter capsulatus | porphobilinogen + 2 H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.6 | in citrate buffer, Tris-maleate buffer, imidazole buffer and Tris buffer | Rhodobacter capsulatus |