Activating Compound | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | half-activation of wild-type at 0.0258 mM, mutant D444N 1.234 mM, mutant S500L 0.063 mM, respectively | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D444N | about 95% of wild-type activity, increase in the basal enzymatic activity in presence of pyridoxal 5'-phosphate | Homo sapiens |
K523Sfs?18 | enzymatic function of the variants is not impaired, increase in the basal enzymatic activity in presence of pyridoxal 5'-phosphate | Homo sapiens |
L540Q | enzymatic function of the variants is not impaired, increase in the basal enzymatic activity in presence of pyridoxal 5'-phosphate | Homo sapiens |
P427L | enzymatic function of the variants is not impaired. Mutant lacks activation by S-adenosyl-L-methionine, but binds it at low level and shows an increase in the basal enzymatic activity in presence of pyridoxal 5'-phosphate | Homo sapiens |
S500L | about 71% of wild-type activity. Mutant lacks activation by S-adenosyl-L-methionine, but binds it at low level and shows an increase in the basal enzymatic activity in presence of pyridoxal 5'-phosphate | Homo sapiens |
V449G | enzymatic function of the variants is not impaired, increase in the basal enzymatic activity in presence of pyridoxal 5'-phosphate | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-homocysteine | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
L-homocysteine | wild-type, 37°C, pH not specified in the publication | Homo sapiens | |
0.31 | - |
L-homocysteine | mutant K523Sfs?18, 37°C, pH not specified in the publication | Homo sapiens | |
0.32 | - |
L-homocysteine | mutant S500L, 37°C, pH not specified in the publication | Homo sapiens | |
0.35 | - |
L-homocysteine | mutant V449G, 37°C, pH not specified in the publication | Homo sapiens | |
0.4 | - |
L-homocysteine | mutant D444N, 37°C, pH not specified in the publication | Homo sapiens | |
0.47 | - |
L-homocysteine | mutant L540Q, 37°C, pH not specified in the publication | Homo sapiens | |
0.69 | - |
L-homocysteine | mutant P427L, 37°C, pH not specified in the publication | Homo sapiens | |
1.9 | - |
L-serine | mutant L540Q, 37°C, pH not specified in the publication | Homo sapiens | |
2 | - |
L-serine | mutant P427L, 37°C, pH not specified in the publication | Homo sapiens | |
2.1 | - |
L-serine | mutant S500L, 37°C, pH not specified in the publication | Homo sapiens | |
2.7 | - |
L-serine | mutant V449G, 37°C, pH not specified in the publication | Homo sapiens | |
2.9 | - |
L-serine | mutant D444N, 37°C, pH not specified in the publication | Homo sapiens | |
3.6 | - |
L-serine | wild-type, 37°C, pH not specified in the publication | Homo sapiens | |
3.7 | - |
L-serine | mutant K523Sfs?18, 37°C, pH not specified in the publication | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
250000 | - |
dynamic light scattering | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P35520 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + L-homocysteine | - |
Homo sapiens | L-cystathionine + H2O | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
L-homocysteine | mutant K523Sfs?18, 37°C, pH not specified in the publication | Homo sapiens | |
7.7 | - |
L-homocysteine | mutant P427L, 37°C, pH not specified in the publication | Homo sapiens | |
12 | - |
L-homocysteine | mutant V449G, 37°C, pH not specified in the publication | Homo sapiens | |
12.8 | - |
L-homocysteine | mutant L540Q, 37°C, pH not specified in the publication | Homo sapiens | |
12.8 | - |
L-homocysteine | wild-type, 37°C, pH not specified in the publication | Homo sapiens | |
13.4 | - |
L-homocysteine | mutant D444N, 37°C, pH not specified in the publication | Homo sapiens | |
27.8 | - |
L-homocysteine | mutant S500L, 37°C, pH not specified in the publication | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
L-serine | wild-type, 37°C, pH not specified in the publication | Homo sapiens | |
0.46 | - |
L-serine | mutant D444N, 37°C, pH not specified in the publication | Homo sapiens | |
0.71 | - |
L-serine | mutant K523Sfs?18, 37°C, pH not specified in the publication | Homo sapiens | |
0.83 | - |
L-serine | mutant V449G, 37°C, pH not specified in the publication | Homo sapiens | |
1.06 | - |
L-serine | mutant S500L, 37°C, pH not specified in the publication | Homo sapiens | |
1.93 | - |
L-serine | mutant P427L, 37°C, pH not specified in the publication | Homo sapiens | |
2.8 | - |
L-homocysteine | mutant D444N, 37°C, pH not specified in the publication | Homo sapiens | |
3 | - |
L-homocysteine | wild-type, 37°C, pH not specified in the publication | Homo sapiens | |
3.61 | - |
L-serine | mutant L540Q, 37°C, pH not specified in the publication | Homo sapiens | |
4.71 | - |
L-homocysteine | mutant V449G, 37°C, pH not specified in the publication | Homo sapiens | |
4.82 | - |
L-homocysteine | mutant S500L, 37°C, pH not specified in the publication | Homo sapiens | |
5.22 | - |
L-homocysteine | mutant K523Sfs?18, 37°C, pH not specified in the publication | Homo sapiens | |
5.23 | - |
L-homocysteine | mutant P427L, 37°C, pH not specified in the publication | Homo sapiens | |
11 | - |
L-homocysteine | mutant L540Q, 37°C, pH not specified in the publication | Homo sapiens |