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Literature summary for 4.2.1.22 extracted from

  • Smith, A.T.; Majtan, T.; Freeman, K.M.; Su, Y.; Kraus, J.P.; Burstyn, J.N.
    Cobalt cystathionine beta-synthase: a cobalt-substituted heme protein with a unique thiolate ligation motif (2011), Inorg. Chem., 50, 4417-4427.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.22

Activating Compound

Activating Compound Comment Organism Structure
S-adenosyl-L-methionine
-
 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ reactivity of Co(III) hCBS with HgCl2 is consistent with a loss of the cysteine(thiolate) ligand. 2-Mercaptoethanol is unable to reverse the Hg-induced ligand switch, in contrast to some other heme-thiolate proteins Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Co3+ cobalt-substituted variant of hCBS, i.e. Co hCBS, in which CoPPIX replaces FePPIX, i.e. heme. Co(III) hCBS is a unique Co-substituted heme protein: the Co(III) ion is 6-coordinate, low-spin, diamagnetic, and bears a cysteine(thiolate) as one of its axial ligands. Electronic absorption and MCD spectra of the Co-substituted heme protein, overview. Co(III) hCBS is slowly reduced to Co(II) hCBS, which contains a 5-coordinate, low-spin, S = 1/2 Co-porphyrin that does not retain the cysteine(thiolate) ligand. This form of Co(II)hCBS binds NO but not CO. Co(II) hCBS is reoxidized in the air to form a new Co(III) form, which does not contain a cysteine(thiolate) ligand. Maintaining the native heme ligation motif of wild-type Fe hCBS (Cys/His) is essential in maintaining maximal activity in Co hCBS Homo sapiens
Fe2+ wild-type FePPIX, i.e. heme Homo sapiens
additional information maintaining the native heme ligation motif of wild-type Fe hCBS (Cys/His) is essential in maintaining maximal activity in Co hCBS Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 a cobalt-substituted variant of hCBS, i.e. Co hCBS, in which CoPPIX replaces FePPIX, i.e. heme
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme performs L-cysteine sythesis from L-serine Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
CBS
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
heme
-
 Homo sapiens
pyridoxal 5'-phosphate
-
 Homo sapiens

General Information

General Information Comment Organism
metabolism CBS is a key enzyme in the trans-sulfuration pathway and catalyzes the condensation of serine with homocysteine to produce cystathionine Homo sapiens