Any feedback?
Literature summary for 4.2.1.22 extracted from
- Kinetic characterization of recombinant human cystathionine beta-synthase purified from E. coli (2009), Protein Expr. Purif., 64, 139-145.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain DH10B | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-homocysteine | substrate inhibition | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
L-cystathionine | recombinant 6-His-tagged enzyme, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens | |
| 0.43 | - |
L-homocysteine | recombinant 6-His-tagged enzyme, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens | |
| 5 | - |
L-serine | recombinant 6-His-tagged enzyme, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
His-tagged enzyme, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.045 | - |
recombinant 6-His-tagged enzyme from crude extract, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens |
| 3.17 | - |
recombinant 6-His-tagged enzyme after purification, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + L-homocysteine | - |
Homo sapiens | L-cystathionine + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CBS | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
L-homocysteine | recombinant 6-His-tagged enzyme, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens | |
| 1.67 | - |
L-serine | recombinant 6-His-tagged enzyme, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
around pH 8.6 | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Homo sapiens | |
| pyridoxal 5'-phosphate | - |
Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.1 | - |
L-homocysteine | recombinant 6-His-tagged enzyme, in 50 mM Tris (pH 8.6), at 25°C | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
