Cloned (Comment) | Organism |
---|---|
fusion protein with glutathione S-transferase | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C52A | reduced heme content, pyridoxal phosphate content comparable to wild-type enzyme, low catalytic activity | Homo sapiens |
C52S | reduced heme content, pyridoxal phosphate content comparable to wild-type enzyme, low catalytic activity | Homo sapiens |
H65R | reduced heme and pyridoxal phosphate content, low catalytic activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Serine + homocysteine | - |
Homo sapiens | Cystathionine + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
wild-type enzyme | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | activity increases with increasing pH, C52S and H65R mutant | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Homo sapiens | |
pyridoxal 5'-phosphate | - |
Homo sapiens |