Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.22 extracted from

  • Ojha, S.; Wu, J.; LoBrutto, R.; Banerjee, R.
    Effects of heme ligand mutations including a pathogenic variant, H65R, on the properties of human cystathionine beta-synthase (2002), Biochemistry, 41, 4649-4654.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
fusion protein with glutathione S-transferase Homo sapiens

Protein Variants

Protein Variants Comment Organism
C52A reduced heme content, pyridoxal phosphate content comparable to wild-type enzyme, low catalytic activity Homo sapiens
C52S reduced heme content, pyridoxal phosphate content comparable to wild-type enzyme, low catalytic activity Homo sapiens
H65R reduced heme and pyridoxal phosphate content, low catalytic activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Serine + homocysteine
-
Homo sapiens Cystathionine + H2O
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
wild-type enzyme Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 activity increases with increasing pH, C52S and H65R mutant Homo sapiens

Cofactor

Cofactor Comment Organism Structure
heme
-
Homo sapiens
pyridoxal 5'-phosphate
-
Homo sapiens