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Literature summary for 4.2.1.20 extracted from

  • Herger, M.; Van Roye, P.; Romney, D.; Brinkmann-Chen, S.; Buller, A.; Arnold, F.
    Synthesis of beta-branched tryptophan analogues using an engineered subunit of tryptophan synthase (2016), J. Am. Chem. Soc., 138, 8388-8391 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.20

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type in complex with L-threonine, to 1.54 A. L-threonine binds non-covalently, no formation of an electrophilic amino-acrylate intermediate. With mutant I16V/E17G/I68V/F95L/F274S/T292S/T321A/V384A, the species is formed Pyrococcus furiosus

Protein Variants

Protein Variants Comment Organism
I16V/E17G/I68V/F95L/F274S/T292S/T321A/V384A significant increase in activity with L-threonine Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U093 beta chain 1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-threonine + 2-methylindole
-
 Pyrococcus furiosus 2-methyl-(2S,3S)-beta-methyltryptophan + H2O
-
 ?
L-threonine + 4-fluoroindole reaction of mutant I16V/E17G/I68V/F95L/F274S/T292S/T321A/V384A Pyrococcus furiosus 4-fluoro-(2S,3S)-beta-methyltryptophan + H2O
-
 ?
L-threonine + 5-fluoroindole reaction of mutant I16V/E17G/I68V/F95L/F274S/T292S/T321A/V384A Pyrococcus furiosus 5-fluoro-(2S,3S)-beta-methyltryptophan + H2O
-
 ?
L-threonine + 6-methylindole reaction of mutant I16V/E17G/I68V/F95L/F274S/T292S/T321A/V384A Pyrococcus furiosus 6-methyl-(2S,3S)-beta-methyltryptophan + H2O
-
 ?
L-threonine + 7-azaindole reaction of mutant I16V/E17G/I68V/F95L/F274S/T292S/T321A/V384A Pyrococcus furiosus 7-aza-(2S,3S)-beta-methyltryptophan + H2O
-
 ?
L-threonine + indole
-
 Pyrococcus furiosus (2S,3S)-beta-methyltryptophan + H2O
-
 ?