Literature summary for 4.2.1.20 extracted from

Caulkins, B.; Young, R.; Kudla, R.; Yang, C.; Bittbauer, T.; Bastin, B.; Hilario, E.; Fan, L.; Marsella, M.; Dunn, M.; Mueller, L.
NMR crystallography of a carbanionic intermediate in tryptophan synthase chemical structure, tautomerization, and reaction specificity (2016), J. Am. Chem. Soc., 138, 15214-15226 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with inhibitor N-(4'-trifluoro-methoxybenzenesulfonyl)-2-amino-ethyl phosphate	Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors	Comment	Organism	Structure
N-(4'-trifluoro-methoxybenzenesulfonyl)-2-amino-ethyl phosphate	tight binding analogue of 1-C-(indol-3-yl)glycerol 3-phosphate which binds to the alpha-subunit	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	P00929	-	-
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720	P00929	-	-

Synonyms

Synonyms	Comment	Organism
TrpA	alpha-chain	Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	residue Lys87 of the beta-subunit activates C4' of pyridoxal 5'-phosphate for substrate nucleophilic attack	Salmonella enterica subsp. enterica serovar Typhimurium

General Information

General Information	Comment	Organism
physiological function	residue Lys87 of the beta subunit plays multiple catalytic roles, it bonds to the pyridoxal phosphate cofactor, activates C4' for nucleophilic attack via a protonated Schiff base nitrogen, and abstracts and returns protons to pyridoxal phosphate-bound substrates. The protonation state of the epsilon-amino group switches between protonated and neutral states as the beta-subunit undergoes conversion from one intermediate to the next during catalysis	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)