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Literature summary for 4.2.1.20 extracted from

  • Blei, F.; Baldeweg, F.; Fricke, J.; Hoffmeister, D.
    Biocatalytic production of psilocybin and derivatives in tryptophan synthase-enhanced reactions (2018), Chemistry, 24, 10028-10031 .
    View publication on PubMed

Application

Application Comment Organism
synthesis use of enzyme in production of psilocybin formation from 4-hydroxyindole and L-serine, and similarly of 7-phosphoryloxytryptamine (isonorbaeocystin), and of serotonin, together with Psilocybe cubensis enzymes PsiD, PsiK, and PsiM, which provide decarboxylase, kinase, and methyltransferase activity, respectively Psilocybe cubensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
4-hydroxyindole pH 7.0, 30°C Psilocybe cubensis
0.04
-
indole pH 7.0, 30°C Psilocybe cubensis
2.9
-
L-serine pH 7.0, 30°C Psilocybe cubensis

Organism

Organism UniProt Comment Textmining
Psilocybe cubensis A0A2U8JPY3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + 4-hydroxyindole
-
Psilocybe cubensis 4-hydroxytryptophan + H2O
-
?
L-serine + 5-hydroxyindole
-
Psilocybe cubensis 5-hydroxytryptophan + H2O
-
?
L-serine + 7-hydroxyindole
-
Psilocybe cubensis 7-hydroxytryptophan + H2O
-
?
L-serine + indole
-
Psilocybe cubensis tryptophan + H2O
-
?
additional information no substrate: 6-hydroxyindole Psilocybe cubensis ?
-
?

Subunits

Subunits Comment Organism
? x * 75500, calculated from sequence Psilocybe cubensis

Synonyms

Synonyms Comment Organism
trpB
-
Psilocybe cubensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Psilocybe cubensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Psilocybe cubensis

pI Value

Organism Comment pI Value Maximum pI Value
Psilocybe cubensis calculated from sequence
-
5.9