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Literature summary for 4.2.1.20 extracted from

  • Busch, F.; Rajendran, C.; Mayans, O.; Loeffler, P.; Merkl, R.; Sterner, R.
    TrpB2 enzymes are O-phospho-L-serine dependent tryptophan synthases (2014), Biochemistry, 53, 6078-6083 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana
expression in Escherichia coli Thermotoga maritima
expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
O-phospho-L-serine pH 7.5, 30°C Arabidopsis thaliana
0.014
-
O-phospho-L-serine isoform TrpB2a, pH 7.5, 60°C Saccharolobus solfataricus
0.015
-
O-phospho-L-serine isoform TrpB2i, pH 7.5, 60°C Saccharolobus solfataricus
0.316
-
O-phospho-L-serine pH 7.5, 80°C Thermotoga maritima
35
-
L-serine pH 7.5, 30°C Arabidopsis thaliana
35
-
L-serine isoform TrpB2i, pH 7.5, 60°C Saccharolobus solfataricus
50.2
-
L-serine pH 7.5, 80°C Thermotoga maritima
151
-
L-serine isoform TrpB2a, pH 7.5, 60°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Saccharolobus solfataricus Q97TX6 beta-subunit
-
Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + indole
-
Arabidopsis thaliana L-tryptophan + H2O
-
?
L-serine + indole
-
Thermotoga maritima L-tryptophan + H2O
-
?
L-serine + indole
-
Saccharolobus solfataricus L-tryptophan + H2O
-
?
additional information TrpB2 subunits of tryptophan synthase are O-phospho-L-serine dependent tryptophan synthases, whereas TrpB1 enzymes catalyze the L-serine dependent synthesis of tryptophan Arabidopsis thaliana ?
-
?
additional information TrpB2 subunits of tryptophan synthase are O-phospho-L-serine dependent tryptophan synthases, whereas TrpB1 enzymes catalyze the L-serine dependent synthesis of tryptophan Thermotoga maritima ?
-
?
additional information TrpB2 subunits of tryptophan synthase are O-phospho-L-serine dependent tryptophan synthases, whereas TrpB1 enzymes catalyze the L-serine dependent synthesis of tryptophan Saccharolobus solfataricus ?
-
?
O-phospho-L-serine + indole
-
Arabidopsis thaliana L-tryptophan + phosphate
-
?
O-phospho-L-serine + indole
-
Thermotoga maritima L-tryptophan + phosphate
-
?
O-phospho-L-serine + indole
-
Saccharolobus solfataricus L-tryptophan + phosphate
-
?

Synonyms

Synonyms Comment Organism
TrpB2a
-
Saccharolobus solfataricus
TrpB2i
-
Saccharolobus solfataricus
TrpB2o
-
Arabidopsis thaliana
TrpB2o
-
Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.015
-
O-phospho-L-serine pH 7.5, 30°C Arabidopsis thaliana
0.015
-
O-phospho-L-serine isoform TrpB2a, pH 7.5, 60°C Saccharolobus solfataricus
0.016
-
L-serine pH 7.5, 30°C Arabidopsis thaliana
0.032
-
L-serine isoform TrpB2a, pH 7.5, 60°C Saccharolobus solfataricus
0.2
-
L-serine isoform TrpB2i, pH 7.5, 60°C Saccharolobus solfataricus
0.3
-
O-phospho-L-serine isoform TrpB2i, pH 7.5, 60°C Saccharolobus solfataricus
0.414
-
O-phospho-L-serine pH 7.5, 80°C Thermotoga maritima
0.44
-
L-serine pH 7.5, 80°C Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0002
-
L-serine isoform TrpB2a, pH 7.5, 60°C Saccharolobus solfataricus
0.00045
-
L-serine pH 7.5, 30°C Arabidopsis thaliana
0.005
-
L-serine isoform TrpB2i, pH 7.5, 60°C Saccharolobus solfataricus
0.0087
-
L-serine pH 7.5, 80°C Thermotoga maritima
1.1
-
O-phospho-L-serine isoform TrpB2a, pH 7.5, 60°C Saccharolobus solfataricus
1.3
-
O-phospho-L-serine pH 7.5, 80°C Thermotoga maritima
1.5
-
O-phospho-L-serine pH 7.5, 30°C Arabidopsis thaliana
20
-
O-phospho-L-serine isoform TrpB2i, pH 7.5, 60°C Saccharolobus solfataricus