Cloned (Comment) | Organism |
---|---|
TSB phylogenetic analysis and tree, functional expression of isozyme AtTSBtype2 in Escherichia coli | Arabidopsis thaliana |
TSB phylogenetic analysis and tree, functional expression of isozyme ZmTSBtype2 in Escherichia coli | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of Attsbtype2 T-DNA insertion mutants,SALK lines 011904, 124293, 082810, and 162268, that show no obvious deviation from the wild-type | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000074 | - |
indole | recombinant isozyme AtTSBtype2, pH 8.2, 30°C | Arabidopsis thaliana | |
0.007 | - |
indole | recombinant isozyme TSB type 2, pH 8.2, 30°C | Zea mays | |
6.2 | - |
L-serine | recombinant isozyme TSB type 2, pH 8.2, 30°C | Zea mays | |
35 | - |
L-serine | recombinant isozyme AtTSBtype2, pH 8.2, 30°C | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FFW8 | isozymes AtTSB1 and AtTSBtype2 | - |
Arabidopsis thaliana Col-0 | Q9FFW8 | isozymes AtTSB1 and AtTSBtype2 | - |
Zea mays | - |
isozyme TSB type 2 | - |
Zea mays B73 | - |
isozyme TSB type 2 | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme AtTSBtype2 from Escherichia coli by nickel affinity chromatography | Arabidopsis thaliana |
recombinant isozyme ZmTSBtype2 from Escherichia coli by nickel affinity chromatography | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | maize isozyme TSB type 2 is transcribed in various tissues | Zea mays | - |
additional information | while AtTSB1 is the predominantly expressed isoform in vegetative tissues, AtTSB1 and AtTSBtype2 reach similar transcript levels in seeds, tissue-specific expression pattern, overview | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + indole | - |
Zea mays | L-tryptophan + H2O | - |
? | |
L-serine + indole | - |
Arabidopsis thaliana | L-tryptophan + H2O | - |
? | |
L-serine + indole | - |
Arabidopsis thaliana Col-0 | L-tryptophan + H2O | - |
? | |
L-serine + indole | - |
Zea mays B73 | L-tryptophan + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tryptophan synthase beta | - |
Zea mays |
tryptophan synthase beta | - |
Arabidopsis thaliana |
TSB | - |
Zea mays |
TSB | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Zea mays |
30 | - |
assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
indole | recombinant isozyme AtTSBtype2, pH 8.2, 30°C | Arabidopsis thaliana | |
0.0102 | - |
indole | recombinant isozyme TSB type 2, pH 8.2, 30°C | Zea mays | |
0.0155 | - |
L-serine | recombinant isozyme AtTSBtype2, pH 8.2, 30°C | Arabidopsis thaliana | |
0.0223 | - |
L-serine | recombinant isozyme TSB type 2, pH 8.2, 30°C | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
assay at | Zea mays |
8.2 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Zea mays | |
pyridoxal 5'-phosphate | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | AtTSBtype2 function is not essential under standard growth conditions | Arabidopsis thaliana |