Cloned (Comment) | Organism |
---|---|
wild-type and P28L/Y173F double mutant alpha-subunits are overexpressed in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
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wild-type and P28L/Y173F double mutant alpha-subunits are crystallized at 25°C by the hanging-drop vapor-diffusion method. X-ray diffraction data are collected to 2.5 A resolution from the wild-type crystals and to 1.8 A from the crystals of the double mutant. The wild-type crystals belonged to the monoclinic space group C2 (a = 155.64 A, b = 44.54 A, c = 71.53 A and beta = 96.39°) and the P28L/Y173F crystals to the monoclinic space group P 2(1) (a = 71.09 A, b = 52.70 A, c = 71.52 A, and beta = 91.49°). The asymmetric unit of both structures contains two molecules of tryptophan synthase alpha-subunit | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
P28L/Y173F | wild-type crystals belonged to the monoclinic space group C2 (a = 155.64 A, b = 44.54 A, c =71.53 A and beta = 96.39°) and the P28L/Y173F crystals to the monoclinic space group P 2(1) (a = 71.09 A, b = 52.70 A, c = 71.52 A, and beta = 91.49°). The asymmetric unit of both structures contains two molecules of tryptophan synthase alpha-subunit | Escherichia coli |
Organism | UniProt | Comment | Textmining |
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Escherichia coli | - |
- |
- |