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Literature summary for 4.2.1.20 extracted from

  • Vadrevu, R.; Falzone, C.J.; Matthews, C.R.
    Partial NMR assignments and secondary structure mapping of the isolated alpha subunit of Escherichia coli tryptophan synthase, a 29-kD TIM barrel protein (2003), Protein Sci., 12, 185-191.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of the alpha-subunit in strain BL21(DE3) as soluble and insoluble protein Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
1 * 29000, alpha-subunit, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
alph-subunit alphaTS
-

Purification (Commentary)

Purification (Comment) Organism
soluble and refolded, solubilized recombinant alpha-subunit from expression in strain BL21(DE3) Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
insoluble rcombinant alpha-subunit, expressed in strain BL212(DE3), is denatured by 6 M urea, refolding Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(indol-3-yl)glycerol 3-phosphate + L-serine
-
Escherichia coli L-tryptophan + D-glyceraldehyde 3-phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 29000, alpha-subunit, SDS-PAGE Escherichia coli
More determination of secondary structure of the isolated alpha-subunit by NMR measurements, the alpha-subunit is a 29 kDa TIM barrel protein, tertiary interactions, overview Escherichia coli
tetramer alpha2beta2 enzyme complex Escherichia coli

Synonyms

Synonyms Comment Organism
alphaTS
-
Escherichia coli