Cloned (Comment) | Organism |
---|---|
expression of the alpha-subunit in strain BL21(DE3) as soluble and insoluble protein | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
1 * 29000, alpha-subunit, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
alph-subunit alphaTS | - |
Purification (Comment) | Organism |
---|---|
soluble and refolded, solubilized recombinant alpha-subunit from expression in strain BL21(DE3) | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) | Escherichia coli |
Renatured (Comment) | Organism |
---|---|
insoluble rcombinant alpha-subunit, expressed in strain BL212(DE3), is denatured by 6 M urea, refolding | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(indol-3-yl)glycerol 3-phosphate + L-serine | - |
Escherichia coli | L-tryptophan + D-glyceraldehyde 3-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29000, alpha-subunit, SDS-PAGE | Escherichia coli |
More | determination of secondary structure of the isolated alpha-subunit by NMR measurements, the alpha-subunit is a 29 kDa TIM barrel protein, tertiary interactions, overview | Escherichia coli |
tetramer | alpha2beta2 enzyme complex | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
alphaTS | - |
Escherichia coli |