Literature summary for 4.2.1.20 extracted from

Weyand, M.; Schlichting, I.
Crystal structure of wild-type tryptophan synthase complexed with the natural substrate indole-3-glycerol phosphate (1999), Biochemistry, 38, 16469-16480.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type enzyme alone or in complex with alpha-subunit substrate analogue indole propanol phosphate, 10 mg/ml in 50 mM bicine, pH 7.8, 10 mM Na-EDTA, 0.02 mM pyridoxal 5'-phosphate, 1 mM dithioerythritol, in the dark at room temperature, hanging drop method, equal volumes of protein and reservoir solution, the latter containing 50 mM bicine, pH 7.8, 5 mM dithioerytritol, 5 mM Na-EDTA, 0.1 mM pyridoxal 5'-phosphate, 2 mM spermine, 2 mM NaN3, and 8-12% w/v PEG 8000, with or without 7 mM indole propanol phosphate, X-ray diffraction structure determination and analysis at 1.4 A resolution, structure modeling	Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Comment)

Organism

purified recombinant wild-type enzyme alone or in complex with alpha-subunit substrate analogue indole propanol phosphate, 10 mg/ml in 50 mM bicine, pH 7.8, 10 mM Na-EDTA, 0.02 mM pyridoxal 5'-phosphate, 1 mM dithioerythritol, in the dark at room temperature, hanging drop method, equal volumes of protein and reservoir solution, the latter containing 50 mM bicine, pH 7.8, 5 mM dithioerytritol, 5 mM Na-EDTA, 0.1 mM pyridoxal 5'-phosphate, 2 mM spermine, 2 mM NaN3, and 8-12% w/v PEG 8000, with or without 7 mM indole propanol phosphate, X-ray diffraction structure determination and analysis at 1.4 A resolution, structure modeling

Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism	UniProt	Comment	Textmining
Salmonella enterica subsp. enterica serovar Typhimurium	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O	also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8)	Salmonella enterica subsp. enterica serovar Typhimurium	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
1-(indol-3-yl)glycerol 3-phosphate	alpha-subunit of the bienzyme complex, alpha-reaction	Salmonella enterica subsp. enterica serovar Typhimurium	D-glyceraldehyde 3-phosphate + indole	-	?
L-serine + indole	beta-subunit of the bienzyme complex, beta-reaction	Salmonella enterica subsp. enterica serovar Typhimurium	L-tryptophan + H2O	-	?
additional information	the intermediate product indole is transferred from the alpha- to the beta-site through a 25 A long hydrophobic tunnel	Salmonella enterica subsp. enterica serovar Typhimurium	?	-	?

Synonyms

Synonyms	Comment	Organism
TRPS	-	Salmonella enterica subsp. enterica serovar Typhimurium