Cloned (Comment) | Organism |
---|---|
overexpression of the alpha-subunit in strain RB797 | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
10 mg/ml purified recombinant alpha-subunit, hanging drop vapour diffusion method, 298K, equal volume, 0.001 ml, of protein solution and reservoir solution are mixed and placed over 0.5 ml reservoir solution, precipitant solution: 0.5 M ammonium sulfate, 0.1 M trisodium citrate dihydrate, 1.0 M lithium sulfate monohydrate, pH 5.6, first crystals after 7-10 days, maximal size within 2 weeks, X-ray diffraction structure determination and analysis at 2.8 A resolution | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant alpha-subunit | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Escherichia coli | D-glyceraldehyde 3-phosphate + indole | channeling of indole to the beta-subunit active site | ? | |
L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Escherichia coli | L-tryptophan + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the alpha-subunit contains no disulfide bond, conformational stabilization mechanism | Escherichia coli |
tetramer | alpha2beta2 enzyme complex | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
alphaTS | alpha-subunit | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the alpha-subunit contains no prosthetic group | Escherichia coli |