Application | Comment | Organism |
---|---|---|
synthesis | overexpression of mutant P160A in Torulopsis glabrata leads to production 5.2 g/l fumarate. Additional deletion of adenylosuccinate synthase, a component of the purine nucleotide cycle, results in production of 9.2 g/l fumarate | Rhizopus arrhizus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhizopus arrhizus |
Protein Variants | Comment | Organism |
---|---|---|
D162K | increase in Km, decrease in kcat/Km value | Rhizopus arrhizus |
D162M | increase in Km, decrease in kcat/Km value | Rhizopus arrhizus |
D162W | decrease in Km and kcat/Km value | Rhizopus arrhizus |
H159S | increase in Km, decrease in kcat/Km value | Rhizopus arrhizus |
H159V | increase in Km, decrease in kcat/Km value | Rhizopus arrhizus |
H159Y | increase in Km, decrease in kcat/Km value | Rhizopus arrhizus |
N161E | decrease in Km and kcat/Km value | Rhizopus arrhizus |
N161F | increase in Km, decrease in kcat/Km value | Rhizopus arrhizus |
N161R | increase in Km, decrease in kcat/Km value | Rhizopus arrhizus |
P160A | decrease in Km value, kcat/Km increases by 33.2% | Rhizopus arrhizus |
P160H | decrease in Km and kcat/Km value | Rhizopus arrhizus |
P160T | decrease in Km and kcat/Km value | Rhizopus arrhizus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.156 | - |
L-malate | mutant N161E, pH 7.3, 30°C | Rhizopus arrhizus | |
0.216 | - |
L-malate | mutant D162W, pH 7.3, 30°C | Rhizopus arrhizus | |
0.268 | - |
L-malate | mutant P160A, pH 7.3, 30°C | Rhizopus arrhizus | |
0.35 | - |
L-malate | mutant P160T, pH 7.3, 30°C | Rhizopus arrhizus | |
0.559 | - |
L-malate | mutant P160H, pH 7.3, 30°C | Rhizopus arrhizus | |
0.574 | - |
L-malate | wild-type, pH 7.3, 30°C | Rhizopus arrhizus | |
0.783 | - |
L-malate | mutant H159S, pH 7.3, 30°C | Rhizopus arrhizus | |
0.797 | - |
L-malate | mutant N161R, pH 7.3, 30°C | Rhizopus arrhizus | |
0.835 | - |
L-malate | mutant D162M, pH 7.3, 30°C | Rhizopus arrhizus | |
0.909 | - |
L-malate | mutant N161F, pH 7.3, 30°C | Rhizopus arrhizus | |
1.08 | - |
L-malate | mutant D162K, pH 7.3, 30°C | Rhizopus arrhizus | |
1.267 | - |
L-malate | mutant H159V, pH 7.3, 30°C | Rhizopus arrhizus | |
1.282 | - |
L-malate | mutant H159Y, pH 7.3, 30°C | Rhizopus arrhizus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizopus arrhizus | P55250 | - |
- |
Rhizopus arrhizus ATCC 10260 | P55250 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-malate | - |
Rhizopus arrhizus | fumarate + H2O | - |
? | |
L-malate | - |
Rhizopus arrhizus ATCC 10260 | fumarate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FUMR | - |
Rhizopus arrhizus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
L-malate | mutant N161E, pH 7.3, 30°C | Rhizopus arrhizus | |
0.011 | - |
L-malate | mutant D162W, pH 7.3, 30°C | Rhizopus arrhizus | |
0.028 | - |
L-malate | mutant P160T, pH 7.3, 30°C | Rhizopus arrhizus | |
0.029 | - |
L-malate | mutant N161F, pH 7.3, 30°C | Rhizopus arrhizus | |
0.033 | - |
L-malate | mutant P160H, pH 7.3, 30°C | Rhizopus arrhizus | |
0.035 | - |
L-malate | mutant P160A, pH 7.3, 30°C | Rhizopus arrhizus | |
0.04 | - |
L-malate | mutant H159S, pH 7.3, 30°C | Rhizopus arrhizus | |
0.045 | - |
L-malate | mutant N161R, pH 7.3, 30°C | Rhizopus arrhizus | |
0.048 | - |
L-malate | mutant H159V, pH 7.3, 30°C | Rhizopus arrhizus | |
0.055 | - |
L-malate | mutant D162M, pH 7.3, 30°C | Rhizopus arrhizus | |
0.055 | - |
L-malate | wild-type, pH 7.3, 30°C | Rhizopus arrhizus | |
0.065 | - |
L-malate | mutant D162K, pH 7.3, 30°C | Rhizopus arrhizus | |
0.098 | - |
L-malate | mutant H159Y, pH 7.3, 30°C | Rhizopus arrhizus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
L-malate | mutant H159Y, pH 7.3, 30°C | Rhizopus arrhizus | |
0.0317 | - |
L-malate | mutant N161F, pH 7.3, 30°C | Rhizopus arrhizus | |
0.0367 | - |
L-malate | mutant H159V, pH 7.3, 30°C | Rhizopus arrhizus | |
0.0433 | - |
L-malate | mutant N161E, pH 7.3, 30°C | Rhizopus arrhizus | |
0.05 | - |
L-malate | mutant D162W, pH 7.3, 30°C | Rhizopus arrhizus | |
0.05 | - |
L-malate | mutant H159S, pH 7.3, 30°C | Rhizopus arrhizus | |
0.055 | - |
L-malate | mutant N161R, pH 7.3, 30°C | Rhizopus arrhizus | |
0.0583 | - |
L-malate | mutant D162K, pH 7.3, 30°C | Rhizopus arrhizus | |
0.0583 | - |
L-malate | mutant P160H, pH 7.3, 30°C | Rhizopus arrhizus | |
0.065 | - |
L-malate | mutant D162M, pH 7.3, 30°C | Rhizopus arrhizus | |
0.08 | - |
L-malate | mutant P160T, pH 7.3, 30°C | Rhizopus arrhizus | |
0.0967 | - |
L-malate | wild-type, pH 7.3, 30°C | Rhizopus arrhizus | |
0.1283 | - |
L-malate | mutant P160A, pH 7.3, 30°C | Rhizopus arrhizus |