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Literature summary for 4.2.1.2 extracted from

  • Chen, X.; Song, W.; Gao, C.; Qin, W.; Luo, Q.; Liu, J.; Liu, L.
    Fumarate production by Torulopsis glabrata engineering heterologous fumarase expression and improving acid tolerance (2016), PLoS ONE, 11, e0164141 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis overexpression of mutant P160A in Torulopsis glabrata leads to production 5.2 g/l fumarate. Additional deletion of adenylosuccinate synthase, a component of the purine nucleotide cycle, results in production of 9.2 g/l fumarate Rhizopus arrhizus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rhizopus arrhizus

Protein Variants

Protein Variants Comment Organism
D162K increase in Km, decrease in kcat/Km value Rhizopus arrhizus
D162M increase in Km, decrease in kcat/Km value Rhizopus arrhizus
D162W decrease in Km and kcat/Km value Rhizopus arrhizus
H159S increase in Km, decrease in kcat/Km value Rhizopus arrhizus
H159V increase in Km, decrease in kcat/Km value Rhizopus arrhizus
H159Y increase in Km, decrease in kcat/Km value Rhizopus arrhizus
N161E decrease in Km and kcat/Km value Rhizopus arrhizus
N161F increase in Km, decrease in kcat/Km value Rhizopus arrhizus
N161R increase in Km, decrease in kcat/Km value Rhizopus arrhizus
P160A decrease in Km value, kcat/Km increases by 33.2% Rhizopus arrhizus
P160H decrease in Km and kcat/Km value Rhizopus arrhizus
P160T decrease in Km and kcat/Km value Rhizopus arrhizus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.156
-
L-malate mutant N161E, pH 7.3, 30°C Rhizopus arrhizus
0.216
-
L-malate mutant D162W, pH 7.3, 30°C Rhizopus arrhizus
0.268
-
L-malate mutant P160A, pH 7.3, 30°C Rhizopus arrhizus
0.35
-
L-malate mutant P160T, pH 7.3, 30°C Rhizopus arrhizus
0.559
-
L-malate mutant P160H, pH 7.3, 30°C Rhizopus arrhizus
0.574
-
L-malate wild-type, pH 7.3, 30°C Rhizopus arrhizus
0.783
-
L-malate mutant H159S, pH 7.3, 30°C Rhizopus arrhizus
0.797
-
L-malate mutant N161R, pH 7.3, 30°C Rhizopus arrhizus
0.835
-
L-malate mutant D162M, pH 7.3, 30°C Rhizopus arrhizus
0.909
-
L-malate mutant N161F, pH 7.3, 30°C Rhizopus arrhizus
1.08
-
L-malate mutant D162K, pH 7.3, 30°C Rhizopus arrhizus
1.267
-
L-malate mutant H159V, pH 7.3, 30°C Rhizopus arrhizus
1.282
-
L-malate mutant H159Y, pH 7.3, 30°C Rhizopus arrhizus

Organism

Organism UniProt Comment Textmining
Rhizopus arrhizus P55250
-
-
Rhizopus arrhizus ATCC 10260 P55250
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-malate
-
Rhizopus arrhizus fumarate + H2O
-
?
L-malate
-
Rhizopus arrhizus ATCC 10260 fumarate + H2O
-
?

Synonyms

Synonyms Comment Organism
FUMR
-
Rhizopus arrhizus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
L-malate mutant N161E, pH 7.3, 30°C Rhizopus arrhizus
0.011
-
L-malate mutant D162W, pH 7.3, 30°C Rhizopus arrhizus
0.028
-
L-malate mutant P160T, pH 7.3, 30°C Rhizopus arrhizus
0.029
-
L-malate mutant N161F, pH 7.3, 30°C Rhizopus arrhizus
0.033
-
L-malate mutant P160H, pH 7.3, 30°C Rhizopus arrhizus
0.035
-
L-malate mutant P160A, pH 7.3, 30°C Rhizopus arrhizus
0.04
-
L-malate mutant H159S, pH 7.3, 30°C Rhizopus arrhizus
0.045
-
L-malate mutant N161R, pH 7.3, 30°C Rhizopus arrhizus
0.048
-
L-malate mutant H159V, pH 7.3, 30°C Rhizopus arrhizus
0.055
-
L-malate mutant D162M, pH 7.3, 30°C Rhizopus arrhizus
0.055
-
L-malate wild-type, pH 7.3, 30°C Rhizopus arrhizus
0.065
-
L-malate mutant D162K, pH 7.3, 30°C Rhizopus arrhizus
0.098
-
L-malate mutant H159Y, pH 7.3, 30°C Rhizopus arrhizus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0075
-
L-malate mutant H159Y, pH 7.3, 30°C Rhizopus arrhizus
0.0317
-
L-malate mutant N161F, pH 7.3, 30°C Rhizopus arrhizus
0.0367
-
L-malate mutant H159V, pH 7.3, 30°C Rhizopus arrhizus
0.0433
-
L-malate mutant N161E, pH 7.3, 30°C Rhizopus arrhizus
0.05
-
L-malate mutant D162W, pH 7.3, 30°C Rhizopus arrhizus
0.05
-
L-malate mutant H159S, pH 7.3, 30°C Rhizopus arrhizus
0.055
-
L-malate mutant N161R, pH 7.3, 30°C Rhizopus arrhizus
0.0583
-
L-malate mutant D162K, pH 7.3, 30°C Rhizopus arrhizus
0.0583
-
L-malate mutant P160H, pH 7.3, 30°C Rhizopus arrhizus
0.065
-
L-malate mutant D162M, pH 7.3, 30°C Rhizopus arrhizus
0.08
-
L-malate mutant P160T, pH 7.3, 30°C Rhizopus arrhizus
0.0967
-
L-malate wild-type, pH 7.3, 30°C Rhizopus arrhizus
0.1283
-
L-malate mutant P160A, pH 7.3, 30°C Rhizopus arrhizus