Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thiol | aerobic purification results in inactive protein, which can only partly be reactivated with Fe2+ and thiol | Escherichia coli O157:H7 |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
expression in Escherichia coli lysY | Escherichia coli |
expression in Escherichia coli lysY | Escherichia coli K-12 |
expression in Escherichia coli lysY | Escherichia coli O157:H7 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.094 | - |
fumarate | pH 6.9, 37°C | Escherichia coli | |
0.094 | - |
fumarate | 37°C, pH 6.9 | Escherichia coli | |
0.1 | - |
mesaconate | pH 6.9, 37°C | Escherichia coli K-12 | |
0.1 | - |
mesaconate | 37°C, pH 6.9 | Escherichia coli | |
0.15 | - |
mesaconate | pH 6.9, 37°C | Escherichia coli O157:H7 | |
0.15 | - |
mesaconate | 37°C, pH 6.9 | Escherichia coli | |
0.2 | - |
(S)-malate | pH 6.9, 37°C | Escherichia coli O157:H7 | |
0.2 | - |
L-malate | 37°C, pH 6.9 | Escherichia coli | |
0.21 | - |
fumarate | pH 6.9, 37°C | Escherichia coli K-12 | |
0.21 | - |
fumarate | 37°C, pH 6.9 | Escherichia coli | |
0.22 | - |
mesaconate | pH 6.9, 37°C | Escherichia coli | |
0.22 | - |
mesaconate | 37°C, pH 6.9 | Escherichia coli | |
0.28 | - |
fumarate | pH 6.9, 37°C | Escherichia coli | |
0.28 | - |
fumarate | 37°C, pH 6.9 | Escherichia coli | |
0.4 | - |
(S)-malate | pH 6.9, 37°C | Escherichia coli | |
0.4 | - |
L-malate | 37°C, pH 6.9 | Escherichia coli | |
0.78 | - |
(S)-malate | pH 6.9, 37°C | Escherichia coli K-12 | |
0.78 | - |
L-malate | 37°C, pH 6.9 | Escherichia coli | |
0.8 | - |
(2S,3S)-tartrate | pH 6.9, 37°C | Escherichia coli K-12 | |
0.8 | - |
(S,S)-Tartrate | 37°C, pH 6.9 | Escherichia coli | |
0.9 | - |
fumarate | pH 6.9, 37°C | Escherichia coli O157:H7 | |
0.9 | - |
fumarate | 37°C, pH 6.9 | Escherichia coli | |
0.92 | - |
(S)-citramalate | pH 6.9, 37°C | Escherichia coli O157:H7 | |
0.92 | - |
(S)-citramalate | 37°C, pH 6.9 | Escherichia coli | |
0.93 | - |
(S)-malate | pH 6.9, 37°C | Escherichia coli | |
0.93 | - |
L-malate | 37°C, pH 6.9 | Escherichia coli | |
1.04 | - |
(S)-citramalate | pH 6.9, 37°C | Escherichia coli K-12 | |
1.04 | - |
(S)-citramalate | 37°C, pH 6.9 | Escherichia coli | |
1.08 | - |
(S)-citramalate | pH 6.9, 37°C | Escherichia coli | |
1.08 | - |
(S)-citramalate | 37°C, pH 6.9 | Escherichia coli | |
2 | - |
(2S,3S)-tartrate | pH 6.9, 37°C | Escherichia coli | |
2 | - |
(S,S)-Tartrate | 37°C, pH 6.9 | Escherichia coli | |
2.6 | - |
(2S,3S)-tartrate | pH 6.9, 37°C | Escherichia coli O157:H7 | |
2.6 | - |
(S,S)-Tartrate | 37°C, pH 6.9 | Escherichia coli | |
6.2 | - |
(2S,3S)-tartrate | pH 6.9, 37°C | Escherichia coli | |
6.2 | - |
(S,S)-Tartrate | 37°C, pH 6.9 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | aerobic purification results in inactive protein, which can only partly be reactivated with Fe2+ and thiol | Escherichia coli O157:H7 | |
additional information | activity does not increase upon incubation with Fe2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Escherichia coli |
60000 | - |
SDS-PAGE | Escherichia coli K-12 |
60000 | - |
SDS-PAGE | Escherichia coli |
60000 | - |
SDS-PAGE | Escherichia coli O157:H7 |
60105 | - |
calculated from sequence | Escherichia coli K-12 |
60105 | - |
calculated from sequence | Escherichia coli O157:H7 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
mesaconate + H2O | Escherichia coli O157:H7 | - |
(S)-citramalate | - |
r | |
mesaconate + H2O | Escherichia coli O157:H7 ATCC 700728 | - |
(S)-citramalate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P05042 | - |
- |
Escherichia coli | P0AC33 | - |
- |
Escherichia coli | P14407 | - |
- |
Escherichia coli | Q8X999 | - |
- |
Escherichia coli K-12 | P14407 | - |
- |
Escherichia coli K-12 W3110 / K-12 | P14407 | - |
- |
Escherichia coli O157:H7 | - |
- |
- |
Escherichia coli O157:H7 ATCC 700728 | - |
- |
- |
Escherichia coli W3110 / K-12 | P05042 | - |
- |
Escherichia coli W3110 / K-12 | P0AC33 | - |
- |
Oxidation Stability | Organism |
---|---|
aerobic purification results in inactive protein, which can only partly be reactivated with Fe2+ and thiol | Escherichia coli O157:H7 |
the enzyme remains stable under oxic conditions | Escherichia coli |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
- |
Escherichia coli K-12 |
- |
Escherichia coli O157:H7 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S)-tartrate | - |
Escherichia coli | oxaloacetate + H2O | - |
? | |
(2S,3S)-tartrate | - |
Escherichia coli K-12 | oxaloacetate + H2O | - |
? | |
(2S,3S)-tartrate | - |
Escherichia coli O157:H7 | oxaloacetate + H2O | - |
? | |
(2S,3S)-tartrate | - |
Escherichia coli K-12 W3110 / K-12 | oxaloacetate + H2O | - |
? | |
(2S,3S)-tartrate | - |
Escherichia coli O157:H7 ATCC 700728 | oxaloacetate + H2O | - |
? | |
(S)-citramalate | - |
Escherichia coli K-12 | mesaconate + H2O | - |
r | |
(S)-citramalate | - |
Escherichia coli | mesaconate + H2O | - |
r | |
(S)-citramalate | - |
Escherichia coli O157:H7 | mesaconate + H2O | - |
r | |
(S)-citramalate | the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate | Escherichia coli | mesaconate + H2O | - |
r | |
(S)-citramalate | - |
Escherichia coli K-12 W3110 / K-12 | mesaconate + H2O | - |
r | |
(S)-malate | - |
Escherichia coli | fumarate + H2O | - |
r | |
(S)-malate | - |
Escherichia coli K-12 | fumarate + H2O | - |
r | |
(S)-malate | - |
Escherichia coli O157:H7 | fumarate + H2O | - |
r | |
(S)-malate | the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate | Escherichia coli | fumarate + H2O | - |
r | |
(S)-malate | - |
Escherichia coli K-12 W3110 / K-12 | fumarate + H2O | - |
r | |
(S)-malate | - |
Escherichia coli W3110 / K-12 | fumarate + H2O | - |
r | |
(S)-malate | - |
Escherichia coli O157:H7 ATCC 700728 | fumarate + H2O | - |
r | |
(S,S)-tartrate | - |
Escherichia coli | oxaloacetate + H2O | - |
? | |
(S,S)-tartrate | - |
Escherichia coli W3110 / K-12 | oxaloacetate + H2O | - |
? | |
fumarate + H2O | - |
Escherichia coli | L-malate | - |
r | |
fumarate + H2O | - |
Escherichia coli W3110 / K-12 | L-malate | - |
r | |
fumarate + H2O | - |
Escherichia coli | (S)-malate | - |
r | |
fumarate + H2O | heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate | Escherichia coli K-12 | (S)-malate | - |
r | |
fumarate + H2O | heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate | Escherichia coli O157:H7 | (S)-malate | - |
r | |
fumarate + H2O | the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate | Escherichia coli | (S)-malate | - |
r | |
fumarate + H2O | heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate | Escherichia coli K-12 W3110 / K-12 | (S)-malate | - |
r | |
fumarate + H2O | heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate | Escherichia coli O157:H7 ATCC 700728 | (S)-malate | - |
r | |
L-malate | - |
Escherichia coli | fumarate + H2O | - |
r | |
L-malate | - |
Escherichia coli W3110 / K-12 | fumarate + H2O | - |
r | |
mesaconate + H2O | - |
Escherichia coli | (S)-citramalate | - |
r | |
mesaconate + H2O | - |
Escherichia coli O157:H7 | (S)-citramalate | - |
r | |
mesaconate + H2O | heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate | Escherichia coli K-12 | (S)-citramalate | - |
r | |
mesaconate + H2O | heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate | Escherichia coli O157:H7 | (S)-citramalate | - |
r | |
mesaconate + H2O | the catalytic efficiency of FumA with (S)-citramalate/mesaconate is about 4% of that with fumarate or (S)-malate | Escherichia coli | (S)-citramalate | - |
r | |
mesaconate + H2O | heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate | Escherichia coli K-12 W3110 / K-12 | (S)-citramalate | - |
r | |
mesaconate + H2O | - |
Escherichia coli O157:H7 ATCC 700728 | (S)-citramalate | - |
r | |
mesaconate + H2O | heterologously produced FumD is a promiscuous mesaconase/fumarase with a 2- to 3-fold preference for mesaconate over fumarate | Escherichia coli O157:H7 ATCC 700728 | (S)-citramalate | - |
r | |
additional information | almost no activity with mesaconate | Escherichia coli | ? | - |
? | |
additional information | enzyme is a promiscuous mesaconase/fumarase with a 2- to 3fold preference for mesaconate over fumarate | Escherichia coli | ? | - |
? | |
additional information | almost no activity with mesaconate | Escherichia coli W3110 / K-12 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Escherichia coli |
? | x * 50000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
FumA | - |
Escherichia coli |
fumarase A | - |
Escherichia coli |
fumarase C | - |
Escherichia coli |
FumB | - |
Escherichia coli |
FumC | - |
Escherichia coli |
FumD | - |
Escherichia coli K-12 |
FumD | - |
Escherichia coli O157:H7 |
mesaconase/fumarase | - |
Escherichia coli K-12 |
mesaconase/fumarase | - |
Escherichia coli O157:H7 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
0.09437 | - |
assay at | Escherichia coli K-12 |
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Escherichia coli O157:H7 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
assay at | Escherichia coli |
6.9 | - |
assay at | Escherichia coli K-12 |
6.9 | - |
assay at | Escherichia coli O157:H7 |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme participates in the methylaspartate pathway of glutamate fermentation as well as in the metabolism of various C5-dicarboxylic acids such as mesaconate or L-threo-beta-methylmalate. fumD is clustered with the key genes for two enzymes of the methylaspartate pathway of glutamate fermentation, glutamate mutase and methylaspartate ammonia lyase, converting glutamate to mesaconate | Escherichia coli K-12 |
metabolism | the enzyme participates in the methylaspartate pathway of glutamate fermentation as well as in the metabolism of various C5-dicarboxylic acids such as mesaconate or L-threo-beta-methylmalate. fumD is clustered with the key genes for two enzymes of the methylaspartate pathway of glutamate fermentation, glutamate mutase and methylaspartate ammonia lyase, converting glutamate to mesaconate | Escherichia coli O157:H7 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
(S,S)-Tartrate | 37°C, pH 6.9 | Escherichia coli | |
0.05 | - |
(2S,3S)-tartrate | pH 6.9, 37°C | Escherichia coli O157:H7 | |
0.065 | - |
(S,S)-Tartrate | 37°C, pH 6.9 | Escherichia coli | |
0.065 | - |
(2S,3S)-tartrate | pH 6.9, 37°C | Escherichia coli | |
0.635 | - |
(S,S)-Tartrate | 37°C, pH 6.9 | Escherichia coli | |
0.635 | - |
(2S,3S)-tartrate | pH 6.9, 37°C | Escherichia coli | |
2.13 | - |
(S,S)-Tartrate | 37°C, pH 6.9 | Escherichia coli | |
2.13 | - |
(2S,3S)-tartrate | pH 6.9, 37°C | Escherichia coli K-12 | |
35 | - |
(S)-citramalate | 37°C, pH 6.9 | Escherichia coli | |
35 | - |
(S)-citramalate | pH 6.9, 37°C | Escherichia coli | |
38 | - |
(S)-citramalate | 37°C, pH 6.9 | Escherichia coli | |
38 | - |
(S)-citramalate | pH 6.9, 37°C | Escherichia coli K-12 | |
110 | - |
(S)-citramalate | 37°C, pH 6.9 | Escherichia coli | |
110 | - |
(S)-citramalate | pH 6.9, 37°C | Escherichia coli O157:H7 | |
250 | - |
mesaconate | 37°C, pH 6.9 | Escherichia coli | |
250 | - |
mesaconate | pH 6.9, 37°C | Escherichia coli | |
260 | - |
L-malate | 37°C, pH 6.9 | Escherichia coli | |
260 | - |
(S)-malate | pH 6.9, 37°C | Escherichia coli O157:H7 | |
370 | - |
L-malate | 37°C, pH 6.9 | Escherichia coli | |
370 | - |
(S)-malate | pH 6.9, 37°C | Escherichia coli K-12 | |
390 | - |
L-malate | 37°C, pH 6.9 | Escherichia coli | |
390 | - |
(S)-malate | pH 6.9, 37°C | Escherichia coli | |
580 | - |
mesaconate | 37°C, pH 6.9 | Escherichia coli | |
580 | - |
mesaconate | pH 6.9, 37°C | Escherichia coli K-12 | |
750 | - |
fumarate | 37°C, pH 6.9 | Escherichia coli | |
750 | - |
fumarate | pH 6.9, 37°C | Escherichia coli O157:H7 | |
870 | - |
L-malate | 37°C, pH 6.9 | Escherichia coli | |
870 | - |
(S)-malate | pH 6.9, 37°C | Escherichia coli | |
2000 | - |
mesaconate | 37°C, pH 6.9 | Escherichia coli | |
2000 | - |
mesaconate | pH 6.9, 37°C | Escherichia coli O157:H7 | |
3120 | - |
fumarate | 37°C, pH 6.9 | Escherichia coli | |
3120 | - |
fumarate | pH 6.9, 37°C | Escherichia coli K-12 | |
3500 | - |
fumarate | 37°C, pH 6.9 | Escherichia coli | |
3500 | - |
fumarate | pH 6.9, 37°C | Escherichia coli | |
6600 | - |
fumarate | 37°C, pH 6.9 | Escherichia coli | |
6600 | - |
fumarate | pH 6.9, 37°C | Escherichia coli |