Literature summary for 4.2.1.2 extracted from

Eprintsev, A.T.; Fedorin, D.N.; Sazonova, O.V.; Igamberdiev, A.U.
Expression and properties of the mitochondrial and cytosolic forms of fumarase in sunflower cotyledons (2018), Plant Physiol. Biochem., 129, 305-309 .

Search for more literature for 4.2.1.2

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0025	-	L-malate	isoform Fum2, pH 7.5, temperature not specified in the publication	Helianthus annuus
0.0125	-	L-malate	isoform Fum1, pH 7.5, temperature not specified in the publication	Helianthus annuus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	about 25% of total activity	Helianthus annuus	5829	-
mitochondrion	about 75% of total activity	Helianthus annuus	5739	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	10fold activation of isoform Fum2	Helianthus annuus
Mg2+	about 20fold activation of isoform Fum1	Helianthus annuus
Mn2+	15fold activation of isoform Fum2	Helianthus annuus
additional information	not activating: Ca2+, K+	Helianthus annuus
additional information	not activating: Ca2+, K+, Mn2+	Helianthus annuus

Organism

Organism	UniProt	Comment	Textmining
Helianthus annuus	A0A251UH44	isoform Fum1	-
Helianthus annuus	A0A251UZF1	isoform Fum2	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cotyledon	-	Helianthus annuus	-
seed	expression of the mitochondrial (Fum1) and cytosolic (Fum2) isoforms increases during germination of sunflower seeds with the peak of the Fum1 gene on the second day and of the Fum2 gene on the third day	Helianthus annuus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.43	-	isoform Fum2, pH 7.5, temperature not specified in the publication	Helianthus annuus
5.97	-	isoform Fum1, pH 7.5, temperature not specified in the publication	Helianthus annuus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-malate	-	Helianthus annuus	fumarate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
FUM1	-	Helianthus annuus
FUM2	-	Helianthus annuus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Helianthus annuus
8	-	-	Helianthus annuus

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Release 2023.1 (January 2023)