Any feedback?
Literature summary for 4.2.1.2 extracted from
- Identification of two [4Fe-4S]-cluster-containing hydro-lyases from Pyrococcus furiosus (2009), Microbiology, 155, 3015-3020 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Pyrococcus furiosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.34 | - |
fumarate | pH 8, 75°C | Pyrococcus furiosus |  |
| 0.41 | - |
(S)-malate | pH 8, 75°C | Pyrococcus furiosus | |
| 0.51 | - |
D-Tartrate | pH 8, 75°C | Pyrococcus furiosus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | [4Fe-4S]-cluster containing heterodimeric enzyme | Pyrococcus furiosus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | Q8U062 and Q8U063 | Q8U062: alpha-subunit, Q8U063: beta-subunit | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate | - |
Pyrococcus furiosus | fumarate + H2O | - |
r | |
| D-tartrate | - |
Pyrococcus furiosus | ? | - |
? | |
| fumarate + H2O | - |
Pyrococcus furiosus | (S)-malate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | - |
Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PF1754 | gene name, PF1755 and PF1754 were shown to form a [4Fe-4S]-clustercontaining heterodimeric enzyme, able to catalyse the reversible hydratation of fumarate | Pyrococcus furiosus |
| PF1755 | gene name, PF1755 and PF1754 were shown to form a [4Fe-4S]-clustercontaining heterodimeric enzyme, able to catalyse the reversible hydratation of fumarate | Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
assay at | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pyrococcus furiosus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| [4Fe-4S]-center | [4Fe-4S]-cluster containing heterodimeric enzyme | Pyrococcus furiosus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme probably plays a role in amino acid synthesis when the organism grows on carbohydrates | Pyrococcus furiosus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 930 | - |
D-Tartrate | pH 8, 75°C | Pyrococcus furiosus | |
| 3200 | - |
fumarate | pH 8, 75°C | Pyrococcus furiosus | |
| 3700 | - |
(S)-malate | pH 8, 75°C | Pyrococcus furiosus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
