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Literature summary for 4.2.1.2 extracted from

  • Eprintsev, A.; Fedorin, D.; Starinina, E.; Igamberdiev, A.
    Expression and properties of the mitochondrial and cytosolic forms of fumarase in germinating maize seeds (2014), Physiol. Plant., 152, 231-240.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
S-malate pH 6.5 cytosolic form, Vmax: 4.5 micromol/min/mg, temperature not specified in the publication Zea mays
0.05
-
S-malate pH 7 mitochondrial, Vmax: 0.3 micromol/min/mg, temperature not specified in the publication Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Zea mays 5829
-
mitochondrion
-
Zea mays 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ cytosolic form gets strongly activated, mitochondrial form only moderately activated Zea mays
Mn2+ cytosolic form gets strongly activated, mitochondrial form only less effectively activated Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using fractionation by (NH4)2SO4, gel filtration of Sephadex G-25 and ion exchange chromatography of DEAE cellulose Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
leaf localisation is only mitochondrial Zea mays
-
scutellum highest fumarase activity detected Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-malate
-
Zea mays fumarate + H2O
-
?

Synonyms

Synonyms Comment Organism
fumarase
-
Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
cytosolic form Zea mays
7
-
mitochondrial form Zea mays