Cloned (Comment) | Organism |
---|---|
entire protein-coding region of fumarase cDNA cloned from Dahl salt-sensitive, SS-13BN, and Brown Norway rats. PCR product inserted into the T-easy vector and propagated in competent Escherichia coli | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
renal medulla | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity of fumarase is 2.9fold higher in SS-13BN rats | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate | - |
Rattus norvegicus | fumarate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
fumarase | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | presence of lysine at amino acid position 481 in Dahl salt-sensitive rats and glutamic acid in Brown Norway and SS-13BN rats. The variation K481E likely contributes to the much higher specific activity of fumarase in SS-13BN rats. Total fumarase activity is significantly lower in the kidneys of Dahl salt-sensitive rats compared with SS-13BN rats, despite an apparent compensatory increase in fumarase abundance in Dahl salt-sensitive rats | Rattus norvegicus |