Crystallization (Comment) | Organism |
---|---|
mutant enzyme E315Q | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E315Q | mutation causes about 3% increase in Km-value for S-malate, about 20% increase in Km-value for fumarate. 10fold decrease in turnover number for S-malate, about 11fold decrease in turnover number for fumarate | Escherichia coli |
additional information | E319Q mutation, an inborn error of fumarase causes progressive psychomotor retardation, failure to thrive, microcephaly and abnormal posture with hypotonia contrasting with hypertonia of limbs | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fumarate + H2O | Homo sapiens | enzyme of Krebs cycle | L-malate | - |
r | |
fumarate + H2O | Escherichia coli | enzyme of Krebs cycle | L-malate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P05042 | - |
- |
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fumarate + H2O | - |
Homo sapiens | L-malate | - |
r | |
fumarate + H2O | - |
Escherichia coli | L-malate | - |
r | |
fumarate + H2O | enzyme of Krebs cycle | Homo sapiens | L-malate | - |
r | |
fumarate + H2O | enzyme of Krebs cycle | Escherichia coli | L-malate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
L-malate | pH 7.9, mutant enzyme E315Q | Escherichia coli | |
1149 | - |
fumarate | pH 7.9, native enzyme | Escherichia coli | |
1150 | - |
fumarate | pH 7.9, native enzyme | Escherichia coli |