Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | - |
Thermus thermophilus |
Application | Comment | Organism |
---|---|---|
synthesis | highly stable enzyme would be ideal for use in various industrial processes, especially since the specific activity is also very high | Thermus thermophilus |
General Stability | Organism |
---|---|
0.8 M guanidine hydrochloride, 50% loss of activity after 5 min at 75°C, complete inactivation at concentrations above 1.5 M | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
(S)-malate | pH 6.5, 50 mM MOPS-NaOH buffer | Thermus thermophilus | |
0.33 | - |
(S)-malate | pH 7.0, 50 mM MOPS-NaOH buffer | Thermus thermophilus | |
1 | - |
(S)-malate | pH 7.5, 50 mM MOPS-NaOH buffer | Thermus thermophilus | |
2 | - |
(S)-malate | pH 6.5, 50 mM potassium phosphate buffer | Thermus thermophilus | |
14 | - |
(S)-malate | pH 7.5, 50 mM potassium phosphate buffer | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
4 * 46000, SDS-PAGE | Thermus thermophilus |
198000 | - |
gel filtration | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Thermus thermophilus | - |
AS-15-1, class II fumarase | - |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1300 | - |
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate | - |
Thermus thermophilus | fumarate + H2O | - |
? | |
(S)-malate | - |
Saccharomyces cerevisiae | fumarate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Saccharomyces cerevisiae |
tetramer | 4 * 46000, SDS-PAGE | Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 100 | 50°C: about 30% of maximal activity, 100°C: about 80% of maximal activity | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
24 h, 10% loss of activity | Thermus thermophilus |
90 | - |
24 h, 20% loss of activity | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Thermus thermophilus |