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Literature summary for 4.2.1.2 extracted from

  • Mizobata, T.; Fujioka, T.; Yamasaki, F.; Hidaka, M.; Nagai, J.; Kawata, Y.
    Purification and characterization of a thermostable class II fumarase from Thermus thermophilus (1998), Arch. Biochem. Biophys., 355, 49-55.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
phosphate
-
Thermus thermophilus

Application

Application Comment Organism
synthesis highly stable enzyme would be ideal for use in various industrial processes, especially since the specific activity is also very high Thermus thermophilus

General Stability

General Stability Organism
0.8 M guanidine hydrochloride, 50% loss of activity after 5 min at 75°C, complete inactivation at concentrations above 1.5 M Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
(S)-malate pH 6.5, 50 mM MOPS-NaOH buffer Thermus thermophilus
0.33
-
(S)-malate pH 7.0, 50 mM MOPS-NaOH buffer Thermus thermophilus
1
-
(S)-malate pH 7.5, 50 mM MOPS-NaOH buffer Thermus thermophilus
2
-
(S)-malate pH 6.5, 50 mM potassium phosphate buffer Thermus thermophilus
14
-
(S)-malate pH 7.5, 50 mM potassium phosphate buffer Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
4 * 46000, SDS-PAGE Thermus thermophilus
198000
-
gel filtration Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Thermus thermophilus
-
AS-15-1, class II fumarase
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1300
-
-
Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate
-
Thermus thermophilus fumarate + H2O
-
?
(S)-malate
-
Saccharomyces cerevisiae fumarate + H2O
-
?

Subunits

Subunits Comment Organism
tetramer
-
Saccharomyces cerevisiae
tetramer 4 * 46000, SDS-PAGE Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Thermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 100 50°C: about 30% of maximal activity, 100°C: about 80% of maximal activity Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
24 h, 10% loss of activity Thermus thermophilus
90
-
24 h, 20% loss of activity Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Thermus thermophilus