Literature summary for 4.2.1.2 extracted from

Keruchenko, J.S.; Keruchenko, I.D.; Gladilin, K.L.; Zaitsev, V.; Chiragadze, N.Y.
Purification, characterization and preliminary X-ray study of fumarase from Saccharomyces cerevisiae (1992), Biochim. Biophys. Acta, 1122, 85-92.

Search for more literature for 4.2.1.2

Activating Compound

Activating Compound	Comment	Organism	Structure
acetate	0.2 M, activation	Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment)	Organism
fumarase complex with meso-tartaric acid	Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50000	-	4 * 50000, SDS-PAGE	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1150	-	-	Saccharomyces cerevisiae

Storage Stability

Storage Stability	Organism
-4°C, stable for at least 6 months	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-malate	-	Saccharomyces cerevisiae	fumarate + H2O	-	r
fumarate + H2O	-	Saccharomyces cerevisiae	L-malate	-	r

Subunits

Subunits	Comment	Organism
tetramer	4 * 50000, SDS-PAGE	Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	complete inactivation within several seconds	Saccharomyces cerevisiae

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4.5	8.2	stable	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)