Literature summary for 4.2.1.18 extracted from

Latimer, S.; Li, Y.; Nguyen, T.T.H.; Soubeyrand, E.; Fatihi, A.; Elowsky, C.G.; Block, A.; Pichersky, E.; Basset, G.J.
Metabolic reconstructions identify plant 3-methylglutaconyl-CoA hydratase that is crucial for branched-chain amino acid catabolism in mitochondria (2018), Plant J., 95, 358-370 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0531	-	(S)-3-hydroxy-3-methylglutaryl-CoA	pH 8.0, 30°C	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Arabidopsis thaliana	5739	-

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	F4JML5	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-3-hydroxy-3-methylglutaryl-CoA	-	Arabidopsis thaliana	trans-3-methylglutaconyl-CoA + H2O	-	?

Synonyms

Synonyms	Comment	Organism
At4g16800	-	Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
23.4	-	(S)-3-hydroxy-3-methylglutaryl-CoA	pH 8.0, 30°C	Arabidopsis thaliana

General Information

General Information	Comment	Organism
physiological function	at4g16800 knockout plants subjected to dark-induced carbon starvation, display accelerated senescence in their rosette leaves as compared with control plants, and a marked increase in the accumulation of free and total leucine, isoleucine and valine. The seeds of the at4g16800 mutant show a similar accumulation of free branched-chain amino acids	Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
440	-	(S)-3-hydroxy-3-methylglutaryl-CoA	pH 8.0, 30°C	Arabidopsis thaliana

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Release 2023.1 (January 2023)