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Literature summary for 4.2.1.17 extracted from

  • Abdel-Mawgoud, A.; Lepine, F.; Deziel, E.
    A chiral high-performance liquid chromatography-tandem mass spectrometry method for the stereospecific analysis of enoyl-coenzyme A hydratases/isomerases (2013), J. Chromatogr. A, 1306, 37-43.
    View publication on PubMed
Search for more literature for 4.2.1.17

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Pseudomonas aeruginosa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 trans-2-decenoyl-CoA + 2 H2O Pseudomonas aeruginosa enzyme activity is of both the ECH-1 and ECH-2 type Pseudomonas aeruginosa (3S)-3-hydroxydecanoyl-CoA + (3R)-3-hydroxydecanoyl-CoA R- and S-enantiomers of produced 3-hydroxydecanoate are nearly equally abundant in case of Pseudomonas aeruginosa ?
additional information development of a chiral high-performance liquid chromatography-tandem mass spectrometry method for analysis of stereospecificity of enoyl-coenzyme A hydratases/isomerases, including reaction of the 3-hydroxyl group on the chiral carbon with 3,5-dimethylphenyl isocyanate, resolving of the resulting urethane derivatives, and monitoring of the liberated free hydroxy fatty acid fragment ion, detailed overview Escherichia coli ?
-
 ?
additional information development of a chiral high-performance liquid chromatography-tandem mass spectrometry method for analysis of stereospecificity of enoyl-coenzyme A hydratases/isomerases, including reaction of the 3-hydroxyl group on the chiral carbon with 3,5-dimethylphenyl isocyanate, resolving of the resulting urethane derivatives, and monitoring of the liberated free hydroxy fatty acid fragment ion, detailed overview Pseudomonas aeruginosa ?
-
 ?
trans-2-decenoyl-CoA + H2O Escherichia coli enzyme activity is of the S-specific ECH-1 type Escherichia coli (3S)-3-hydroxydecanoyl-CoA the distribution of R- and S-enantiomers of produced 3-hydroxydecanoate is in favour of the S-enantiomer in case of Escherichia coli ?
trans-2-decenoyl-CoA + H2O Escherichia coli enzyme activity is of the S-specific ECH-1 type Escherichia coli (3R)-3-hydroxydecanoyl-CoA the distribution of R- and S-enantiomers of produced 3-hydroxydecanoate is in favour of the S-enantiomer in case of Escherichia coli ?

Synonyms

Synonyms Comment Organism
ECH-1 S-specific Escherichia coli
ECH-1 S-specific Pseudomonas aeruginosa
ECH-2 R-specific Escherichia coli
ECH-2 R-specific Pseudomonas aeruginosa
R-3-hydroxyacyl-CoA enoyl-CoA hydratase
-
 Pseudomonas aeruginosa
R-3-hydroxyacyl-CoA enoyl-CoA hydratases
-
 Escherichia coli
S-3-hydroxyacyl-CoA enoyl-CoA hydratase
-
 Pseudomonas aeruginosa
S-3-hydroxyacyl-CoA enoyl-CoA hydratases
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli
30
-
 assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli
8
-
 assay at Pseudomonas aeruginosa