Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
E144A | kcat for trans-2-hexenoyl-CoA is 1733fold lower than wild-type value | Rattus norvegicus |
E144A/Q162L | kcat for trans-2-hexenoyl-CoA is 12417fold lower than wild-type value. The point mutations E144A and Q162L by themselves apparently do not cause structural rearrangements of the active site helix, but when both residues are changed, the active site geometry changes | Rattus norvegicus |
E164A | kcat for trans-2-hexenoyl-CoA is 1709fold lower than wild-type value | Rattus norvegicus |
Q162A | kcat for trans-2-hexenoyl-CoA is nearly identical to wild-type value | Rattus norvegicus |
Q162L | kcat for trans-2-hexenoyl-CoA is nearly identical to wild-type value | Rattus norvegicus |
Q162M | kcat for trans-2-hexenoyl-CoA is nearly identical to wild-type value | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
trans-2-decenoyl-CoA | pH 8.0, 22°C, mutant enzyme E144A/Q162L | Rattus norvegicus | |
0.0029 | - |
trans-2-decenoyl-CoA | pH 8.0, 22°C, mutant enzyme Q162A | Rattus norvegicus | |
0.003 | - |
trans-2-decenoyl-CoA | pH 8.0, 22°C, mutant enzyme E144A | Rattus norvegicus | |
0.0039 | - |
trans-2-decenoyl-CoA | pH 8.0, 22°C, wild-type enzyme | Rattus norvegicus | |
0.005 | - |
trans-2-decenoyl-CoA | pH 8.0, 22°C, mutant enzyme Q162L | Rattus norvegicus | |
0.0052 | - |
trans-2-decenoyl-CoA | pH 8.0, 22°C, mutant enzyme E164A | Rattus norvegicus | |
0.0063 | - |
trans-2-decenoyl-CoA | pH 8.0, 22°C, mutant enzyme Q162M | Rattus norvegicus | |
0.0143 | - |
trans-2-hexenoyl-CoA | pH 8.0, 22°C, mutant enzyme Q162A | Rattus norvegicus | |
0.0152 | - |
trans-2-hexenoyl-CoA | pH 8.0, 22°C, mutant enzyme E144A | Rattus norvegicus | |
0.021 | - |
trans-2-hexenoyl-CoA | pH 8.0, 22°C, mutant enzyme E144A/Q162L | Rattus norvegicus | |
0.0229 | - |
trans-2-hexenoyl-CoA | pH 8.0, 22°C, mutant enzyme Q162M | Rattus norvegicus | |
0.024 | - |
trans-2-hexenoyl-CoA | pH 8.0, 22°C, mutant enzyme E164A | Rattus norvegicus | |
0.025 | - |
trans-2-hexenoyl-CoA | pH 8.0, 22°C, wild-type enzyme | Rattus norvegicus | |
0.027 | - |
trans-2-hexenoyl-CoA | pH 8.0, 22°C, mutant enzyme Q162L | Rattus norvegicus | |
0.0499 | - |
crotonyl-CoA | pH 8.0, 22°C, wild-type enzyme | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
64 | - |
homogenate of cells transformed with the pET3aECH1 expression vector | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + H2O | - |
Rattus norvegicus | (3S)-3-hydroxybutanoyl-CoA | - |
? | |
additional information | the enzyme also catalyzes DELTA3-DELTA2-isomerization of trans-3-hexenoyl-CoA | Rattus norvegicus | ? | - |
? | |
trans-2-decenoyl-CoA + H2O | - |
Rattus norvegicus | (3S)-hydroxydecanoyl-CoA | - |
? | |
trans-2-hexenoyl-CoA + H2O | - |
Rattus norvegicus | (3S)-3-hydroxyhexanoyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-enoyl-CoA hydratase-1 | - |
Rattus norvegicus |
crotonase | - |
Rattus norvegicus |
mECH-1 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0121 | - |
trans-2-decenoyl-CoA | pH 8.0, 22°C, mutant enzyme E144A/Q162L | Rattus norvegicus | |
0.06 | - |
trans-2-hexenoyl-CoA | pH 8.0, 22°C, mutant enzyme E144A/Q162L | Rattus norvegicus | |
0.085 | - |
trans-2-decenoyl-CoA | pH 8.0, 22°C, mutant enzyme E144A | Rattus norvegicus | |
0.095 | - |
trans-2-decenoyl-CoA | pH 8.0, 22°C, mutant enzyme E164A | Rattus norvegicus | |
0.43 | - |
trans-2-hexenoyl-CoA | pH 8.0, 22°C, mutant enzyme E144A | Rattus norvegicus | |
0.44 | - |
trans-2-hexenoyl-CoA | pH 8.0, 22°C, mutant enzyme E164A | Rattus norvegicus | |
104 | - |
trans-2-decenoyl-CoA | pH 8.0, 22°C, mutant enzyme Q162A | Rattus norvegicus | |
164 | - |
trans-2-decenoyl-CoA | pH 8.0, 22°C, mutant enzyme Q162M | Rattus norvegicus | |
174 | - |
trans-2-decenoyl-CoA | pH 8.0, 22°C, mutant enzyme Q162L | Rattus norvegicus | |
203 | - |
trans-2-decenoyl-CoA | pH 8.0, 22°C, wild-type enzyme | Rattus norvegicus | |
561 | - |
trans-2-hexenoyl-CoA | pH 8.0, 22°C, mutant enzyme Q162L | Rattus norvegicus | |
601 | - |
trans-2-hexenoyl-CoA | pH 8.0, 22°C, mutant enzyme Q162M | Rattus norvegicus | |
607 | - |
trans-2-hexenoyl-CoA | pH 8.0, 22°C, mutant enzyme Q162A | Rattus norvegicus | |
745 | - |
trans-2-hexenoyl-CoA | pH 8.0, 22°C, wild-type enzyme | Rattus norvegicus | |
2238 | - |
crotonyl-CoA | pH 8.0, 22°C, wild-type enzyme | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rattus norvegicus |