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Literature summary for 4.2.1.17 extracted from

  • Fong, J.C.; Schulz, H.
    Purification and properties of pig heart crotonase and the presence of short chain and long chain enoyl coenzyme A hydratases in pig and guinea pig tissues (1977), J. Biol. Chem., 252, 542-547.
    View publication on PubMed
Search for more literature for 4.2.1.17

Inhibitors

Inhibitors Comment Organism Structure
acetoacetyl-CoA
-
 Sus scrofa
iodoacetamide 10 mM, 20 min, 20% inhibition Sus scrofa
NEM 10 mM, 19% inhibition. 5 mM, 13% inhibition Sus scrofa
p-chloromercuribenzoate 1 mM, complete inhibition. 0.1 mM, 11% inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 crotonyl-CoA pH 9.0 Sus scrofa
0.029
-
 hex-2-enoyl-CoA pH 9.0 Sus scrofa
0.029
-
 oct-2-enoyl-CoA pH 9.0 Sus scrofa
0.029
-
 dec-2-enoyl-CoA pH 9.0 Sus scrofa
0.03
-
 dodec-2-enoyl-CoA pH 9.0 Sus scrofa
0.03
-
 hexadec-2-enoyl-CoA pH 9.0 Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Sus scrofa 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27300
-
 6 * 27300, SDS-PAGE Sus scrofa
155000
-
 non-denaturing PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
 The classification is ambiguous because the stereochemistry of the reaction product is not exactly determined
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1334
-
-
 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonyl-CoA + H2O best substrate. The classification is ambiguous because the stereochemistry is not exactly determined Sus scrofa ?
-
 ?
dec-2-enoyl-CoA + H2O 32% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Sus scrofa ?
-
 ?
dodec-2-enoyl-CoA + H2O 9.6% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Sus scrofa ?
-
 ?
hex-2-enoyl-CoA + H2O 77% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Sus scrofa ?
-
 ?
hexadec-2-enoyl-CoA + H2O 2.4% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Sus scrofa ?
-
 ?
oct-2-enoyl-CoA + H2O 54% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined Sus scrofa ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 27300, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
crotonase the classification is ambiguous because the stereochemistry of the reaction product is not exactly determined Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.014
-
 acetoacetyl-CoA pH 8.0, due to the binding of the enolate form of acetoacetyl-CoA the Ki-value should be highly pH-dependent Sus scrofa