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Literature summary for 4.2.1.17 extracted from
- Enoyl coenzyme A hydratase (crotonase). Catalytic properties of crotonase and its possible regulatory role in fatty acid oxidation (1972), J. Biol. Chem., 247, 5258-5265.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetoacetyl-CoA | enolate form of acetoacetyl-CoA acts as a competitive inhibitor. 6 molecules of inhibitor are bound per molecule of enzyme | Bos taurus |  |
| decenoyl-CoA | - |
Bos taurus | |
| hexadecenoyl-CoA | - |
Bos taurus | |
| hexenoyl-CoA | - |
Bos taurus | |
| additional information | no inhibition: ethyl acetoacetate, acetoacetate | Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Bos taurus | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
The classification is ambiguous because the stereochemistry of the reaction product is not exactly determined | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E)-octenoyl-CoA + H2O | 36% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined | Bos taurus | ? | - |
? | |
| crotonyl-CoA + H2O | best substrate. The classification is ambiguous because the stereochemistry is not exactly determined | Bos taurus | ? | - |
? | |
| decenoyl-CoA + H2O | 17% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined | Bos taurus | ? | - |
? | |
| dodecenoyl-CoA + H2O | 7% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined | Bos taurus | ? | - |
? | |
| hexadecenoyl-CoA + H2O | 1% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined | Bos taurus | ? | - |
? | |
| hexenoyl-CoA + H2O | 67% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined | Bos taurus | ? | - |
? | |
| tetradecenoyl-CoA + H2O | 2% of the activity with crotonyl-CoA. The classification is ambiguous because the stereochemistry is not exactly determined | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | - |
Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| crotonase | the classification is ambiguous because the stereochemistry is not exactly determined | Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5667 | - |
crotonyl-CoA | pH 7.5, 25°C | Bos taurus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00024 | - |
hexenoyl-CoA | pH 7.5, 25°C | Bos taurus | |
| 0.00028 | - |
octenoyl-CoA | pH 7.5, 25°C | Bos taurus | |
| 0.0003 | - |
decenoyl-CoA | pH 7.5, 25°C | Bos taurus | |
| 0.0004 | - |
dodecenoyl-CoA | pH 7.5, 25°C | Bos taurus | |
| 0.00042 | - |
tetradecenoyl-CoA | pH 7.5, 25°C | Bos taurus | |
| 0.0005 | - |
hexadecenoyl-CoA | pH 7.5, 25°C | Bos taurus | |
| 0.0016 | - |
acetoacetyl-CoA | pH 7.5, 25°C | Bos taurus | |
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Release 2023.1 (January 2023)
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