Literature summary for 4.2.1.158 extracted from

Rakus, J.F.; Kalyanaraman, C.; Fedorov, A.A.; Fedorov, E.V.; Mills-Groninger, F.P.; Toro, R.; Bonanno, J.; Bain, K.; Sauder, J.M.; Burley, S.K.; Almo, S.C.; Jacobson, M.P.; Gerlt, J.A.
Computation-facilitated assignment of the function in the enolase superfamily: a regiochemically distinct galactarate dehydratase from Oceanobacillus iheyensis (2009), Biochemistry, 48, 11546-11558.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
wild-type and mutant Y90F. A Tyr 164-Arg 162 dyad is the base that initiates the reaction by abstraction of the alpha-proton and Tyr 90 is the acid that facilitates departure of the beta-OH leaving group. The structure contains two Mg2+ ions located 10.4 A from one another, with one located in the canonical position in the (beta/alpha)7beta-barrel, the second is located in a site within the capping domain	Oceanobacillus iheyensis

Crystallization (Comment)

Organism

wild-type and mutant Y90F. A Tyr 164-Arg 162 dyad is the base that initiates the reaction by abstraction of the alpha-proton and Tyr 90 is the acid that facilitates departure of the beta-OH leaving group. The structure contains two Mg2+ ions located 10.4 A from one another, with one located in the canonical position in the (beta/alpha)7beta-barrel, the second is located in a site within the capping domain

Oceanobacillus iheyensis

Protein Variants

Protein Variants	Comment	Organism
Y90F	catalytically impaired	Oceanobacillus iheyensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.62	-	galactarate	pH 8.0, 30°C	Oceanobacillus iheyensis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	structure contains two Mg2+ ions	Oceanobacillus iheyensis

Organism

Organism	UniProt	Comment	Textmining
Oceanobacillus iheyensis	Q8EMJ9	-	-
Oceanobacillus iheyensis DSM 14371	Q8EMJ9	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
galactarate	-	Oceanobacillus iheyensis	2-oxo-D-threo-4,5-dihydroxyadipate + H2O	i.e. (2S,3R)-2,3-dihydroxy-5-oxohexanedioate. Product is the enantiomer of the product obtained in the reaction catalyzed by bifunctional L-talarate/galactarate dehydratase. When the GalrD-II-catalyzed reaction is performed in D2O, the proS hydrogen is replaced with deuterium, i.e., the acid that facilitates the departure of the OH group is likely the acid that catalyzes the formation of the alpha-oxo-beta-methylene product	?
galactarate	-	Oceanobacillus iheyensis DSM 14371	2-oxo-D-threo-4,5-dihydroxyadipate + H2O	i.e. (2S,3R)-2,3-dihydroxy-5-oxohexanedioate. Product is the enantiomer of the product obtained in the reaction catalyzed by bifunctional L-talarate/galactarate dehydratase. When the GalrD-II-catalyzed reaction is performed in D2O, the proS hydrogen is replaced with deuterium, i.e., the acid that facilitates the departure of the OH group is likely the acid that catalyzes the formation of the alpha-oxo-beta-methylene product	?

Synonyms

Synonyms	Comment	Organism
galactarate dehydratase (D-threo-forming)	-	Oceanobacillus iheyensis
GalrD-II	-	Oceanobacillus iheyensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
6.8	-	galactarate	pH 8.0, 30°C	Oceanobacillus iheyensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
11	-	galactarate	pH 8.0, 30°C	Oceanobacillus iheyensis