Cloned (Comment) | Organism |
---|---|
cloned into the Escherichia coli expression vector pET28a encoding a N-terminal His6-tag | Streptomyces vinaceus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S)-3-hydroxyarginine | Streptomyces vinaceus | the enzyme is involved in the biosynthesis of the cyclic pentapeptide antibiotic viomycin | (2S,3R)-capreomycidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces vinaceus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces vinaceus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S,3S)-3-hydroxyarginine | the enzyme is involved in the biosynthesis of the cyclic pentapeptide antibiotic viomycin | Streptomyces vinaceus | (2S,3R)-capreomycidine | - |
? | |
(2S,3S)-3-hydroxyarginine | the enzyme catalyzes an intramolecular beta-replacement/elimination reaction. VioD is completely stereospecific for (3S)-hydroxy-L-Arg, as no product formation is observed when (3R)-hydroxy-L-Arg is evaluated as substrate | Streptomyces vinaceus | (2S,3R)-capreomycidine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
VioD | ambiguous | Streptomyces vinaceus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces vinaceus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Streptomyces vinaceus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | pyridoxal 5'-phosphate dependent enzyme. It is not known if the cofactor is covalently bound to VioD through a lysyl imine, as is common with many PLP-dependent enzymes | Streptomyces vinaceus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in the biosynthesis of the cyclic pentapeptide antibiotic viomycin | Streptomyces vinaceus |