Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EGTA | enzyme activity is reduced to 24% after dialysis in buffer containing EGTA. Activity is completely recovered in the presence of 10 mM MgCl2 and recovered to 39% and 62% in the presence of MnCl2 and CoCl2, respectively | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.81 | - |
D-galactonate | pH 6.0, 70°C | Saccharolobus solfataricus | |
1.57 | - |
D-gluconate | pH 6.0, 70°C | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ has a catalytic role | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44729 | - |
1 * 44729, calculated from sequence | Saccharolobus solfataricus |
45000 | - |
1 * 45000, SDS-PAGE | Saccharolobus solfataricus |
48000 | - |
gel filtration | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactonate | Saccharolobus solfataricus | the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the EntnerDoudoroff pathway | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
D-galactonate | Saccharolobus solfataricus P2 | the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the EntnerDoudoroff pathway | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
D-gluconate | Saccharolobus solfataricus | the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the EntnerDoudoroff pathway | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
D-gluconate | Saccharolobus solfataricus P2 | the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the EntnerDoudoroff pathway | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97U27 | - |
- |
Saccharolobus solfataricus P2 | Q97U27 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O | (2) | Saccharolobus solfataricus | |
D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H2O | (1) | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
13.4 U/mg, pH 6.0, 70°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactonate | the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the EntnerDoudoroff pathway | Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
D-galactonate | the catalytic efficiency (kcat/Km) for D-gluconate is 5fold higher compared to the catalytic efficiency for D-galactonate | Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
D-galactonate | the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the EntnerDoudoroff pathway | Saccharolobus solfataricus P2 | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
D-galactonate | the catalytic efficiency (kcat/Km) for D-gluconate is 5fold higher compared to the catalytic efficiency for D-galactonate | Saccharolobus solfataricus P2 | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
D-gluconate | the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the EntnerDoudoroff pathway | Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
D-gluconate | the catalytic efficiency (kcat/Km) for D-gluconate is 5fold higher compared to the catalytic efficiency for D-galactonate | Saccharolobus solfataricus | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
D-gluconate | the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the EntnerDoudoroff pathway | Saccharolobus solfataricus P2 | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
D-gluconate | the catalytic efficiency (kcat/Km) for D-gluconate is 5fold higher compared to the catalytic efficiency for D-galactonate | Saccharolobus solfataricus P2 | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Saccharolobus solfataricus |
monomer | 1 * 44729, calculated from sequence | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
Sso3198 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus solfataricus |
100 | - |
above | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
half-life: 41 min | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.08 | - |
D-galactonate | pH 6.0, 70°C | Saccharolobus solfataricus | |
10.4 | - |
D-gluconate | pH 6.0, 70°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Saccharolobus solfataricus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in glucose and galactose catabolism via a nonphosphorylative variant of the EntnerDoudoroff pathway | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.33 | - |
D-galactonate | pH 6.0, 70°C | Saccharolobus solfataricus | |
6.65 | - |
D-gluconate | pH 6.0, 70°C | Saccharolobus solfataricus |